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Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
BackgroundSalmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking.ResultsThe stability of sixExpand
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Characterisation of salmon and trout CD8alpha and CD8beta.
The genes and corresponding cDNAs of both alpha and beta chains of the Atlantic salmon (Salmo salar) CD8 molecule have been sequenced and characterized. In addition, the cDNAs for alpha and betaExpand
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Transcriptional responses to temperature and low oxygen stress in Atlantic salmon studied with next-generation sequencing technology
BackgroundWarmer seawater as a result of climate change may impose environmental challenges for Atlantic salmon aquaculture in its southernmost geographic range. Seawater temperatures above optimalExpand
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Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua
BackgroundExtensive sequencing efforts have been taking place for the Atlantic cod (Gadus morhua) in recent years, the number of ESTs in the Genbank has reached more than 140.000. Despite itsExpand
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Characterisation of salmon and trout CD8α and CD8β
The genes and corresponding cDNAs of both alpha and beta chains of the Atlantic salmon (Salmo salar) CD8 molecule have been sequenced and characterized. In addition, the cDNAs for alpha and betaExpand
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Molecular cloning of IgT from Atlantic salmon, and analysis of the relative expression of τ, μ, and δ in different tissues.
In the present study, IgT genes of Atlantic salmon were cloned and characterised. Analysis of our sequence data as well as ESTs reported to the databases revealed three distinct IgT heavy chainExpand
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Gene expression profiling in Atlantic cod (Gadus morhua L.) from two contaminated sites using a custom-made cDNA microarray.
A small-scale, custom-made cDNA microarray, the CodStress array, consisting of 746 expressed sequence tag clones encoding stress-responsive and immune-relevant proteins was constructed to investigateExpand
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Transcriptional responses in juvenile Atlantic cod (Gadus morhua) after exposure to mercury-contaminated sediments obtained near the wreck of the German WW2 submarine U-864, and from Bergen Harbor,
The main aim of the present work was to investigate the effects of mercury (Hg)-enriched sediments on fish. Sediments near the sunken German WW2 submarine U-864, which according to historicalExpand
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Transcriptional evidence for low contribution of oil droplets to acute toxicity from dispersed oil in first feeding Atlantic cod (Gadus morhua) larvae.
We evaluated the potential contribution of oil droplets to the toxicity of dispersed oil to first feeding fish larvae. Atlantic cod larvae were exposed to five concentrations of either artificiallyExpand
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Pathogen recognition and mechanisms in Atlantic cod (Gadus morhua) head kidney cells: bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes.
Understanding pathogen recognition and mechanisms in Atlantic cod are of significant importance for both basic research on wild populations and health management in aquaculture. A microarray approachExpand
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