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High‐Throughput Process Development: Determination of Dynamic Binding Capacity Using Microtiter Filter Plates Filled with Chromatography Resin
The proposed high‐throughput method for determination of dynamic binding capacity significantly reduces time and sample consumption as compared to a traditional method utilizing packed chromatography columns without sacrificing the accuracy of data obtained. Expand
Ion exchange chromatography of antibody fragments
Confocal scanning laser microscopy studies indicate increases in Fab dynamic binding capacity were due to faster intra‐particle mass transport and hence greater utilization of the media's available binding capacity, in agreement with recent studies related to ion exchange of whole antibody molecules under similar conditions. Expand
Modeling the enzymatic transformation of 3,5‐dimethoxy,4‐hydroxy cinnamic acid by polyphenoloxidase from the white‐rot fungus Trametes versicolor
It is shown that, in the open system, the observed change in the enzyme activity at higher temperatures was caused by two opposing phenomena: an Arrhenius effect which increased the rate, and a solubility effect which reduced the rate due to a lower oxygen concentration. Expand
High‐throughput techniques to evaluate the effect of ligand density for impurity separations with multimodal cation exchange resins
Scale‐down, high‐throughput screening techniques are well on their way to becoming a commodity in downstream bioprocess development, especially for the rapid development of chromatography processExpand
Changing manufacturing paradigms in downstream processing and the role of alternative bioseparation technologies
BACKGROUND This study ascertains the degree to which bioseparation techniques, considered as alternatives to packed bed chromatography, have been adopted for large-scale biomanufacturing. SuchExpand
Enzymatic transformation of sinapine using polyphenol oxidase from Trametes versicolor. Effect of pH
The results showed that the investigatedenzymatic system includes additional enzymatic reactions between oxygen and the products of sinapine transformation, and the optimum pH of reaction is 4.24 and 50 °C. Expand
High‐throughput process development: Chromatography media volume definition
Prediction of chromatographic separations in liter scale with the help of data gathered using a microliter format is challenging, as both scale effects and differences in the formats used (plates vs.Expand
Stability of a polyphenol oxidase from the white-rot fungus Trametes versicolor in the presence of canola meal
The stability of a polyphenol oxidase (PPO) preparation from the white-rot fungus Trametes versicolor during a process for the enzymatic decrease of the phenolic content of commercial canola mealExpand
Definition and dynamic control of a continuous chromatography process independent of cell culture titer and impurities.
The strategy and results presented in this paper show column loading in a continuous chromatography step can be dynamically controlled independent of the cell culture feedstock and titer, and allow for enhanced process control built into the downstream continuous operations. Expand
Advanced Analysis of Biosensor Data for SARS-CoV-2 RBD and ACE2 Interactions
The results showed that in both cases the standard assumption about a single interaction was erroneous, and in one of the cases the value of the affinity constant KD differed more than 300% between the reported value and the calculation. Expand