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Mechanism of asymmetric production of L-aromatic amino acids from the corresponding hydantoins by Flavobacterium sp.
The mechanism of asymmetric production of l-aroniatic amino acids from the corresponding hydantoins by Flavobacterium sp. AJ-3912 was examined by investigating the properties of the enzymes involved… Expand
Overproduction of Microbial Transglutaminase in Escherichia coli, In Vitro Refolding, and Characterization of the Refolded Form
- K. Yokoyama, N. Nakamura, K. Seguro, K. Kubota
- Medicine, Biology
- Bioscience, biotechnology, and biochemistry
- 1 January 2000
The Streptoverticillium transglutaminase (MTG) gene, synthesized previously for yeast expression, was modified and resynthesized for overexpession in E. coli. A high-level expression plasmid,… Expand
Enzymatic Production of l-Amino Acids from the Corresponding 5-Substituted Hydantoins by a Newly Isolated Bacterium, Bacillus brevis AJ-12299
A bacterium that stereospecifically produces l-valine from 5-isopropylhydantoin was isolated + from soil. It was identified as Bacillus brevis and given the number AJ-12299. l-Valine productivity… Expand
Enzymatic production of D-amino acids from 5-substituted hydantoins. part III Mechanism of asymmetric production of D-amino acids from the corresponding hydantoins by Pseudomonas sp.
Mechanism of Asymmetric Production of D-Amino Acids from the Corresponding Hydantoins by Pseudomonas sp.(Microbiology & Fermentation Industry)
The mechanism of asymmetric production of d-amino acids from the corresponding hydantoins by Pseudomonas sp. AJ-11220 was examined by investigating the properties of the enzymes involved in the… Expand
Production of L-serine from Glycine by Corynebacterium glycinophilum and Properties of Serine Hydroxymethyltransferase, a Key Enzyme in L-Serine Production
Abstract Fermentative production of l -serine from glycine by Corynebacterium glycinophilum AJ-3413, an auxotrophic mutant of Leu and Met with increased productivity of l -serine using a one liter… Expand
Mechanism of Stereospecific Production of L-Amino Acids from the Corresponding 5-Substituted Hydantoins by Bacillus brevis(Microbiology & Fermentation Industry)
The mechanism of stereospecific production of l-amino acids from the corresponding 5-substituted hydantoins by Bacillus brevis AJ-12299 was studied. The enzymes involved in the reaction were… Expand
The molecular assembly of the ionic liquid/aliphatic carboxylic acid/aliphatic amine as effective and safety transdermal permeation enhancers.
- K. Kubota, A. Shibata, Toshikazu Yamaguchi
- Chemistry, Medicine
- European journal of pharmaceutical sciences…
- 30 April 2016
In spite of numerous advantages, transdermal drug delivery systems are unfeasible for most drugs because of the barrier effect of the stratum corneum. Ionic liquids were recently used to enhance… Expand
Enzymatic production of L-tryptophan from DL-5-indolylmethylhydantoin. part IV Mechanism of asymmetric production of L-aromatic amino acids from the corresponding hydantoins by Flavobacterium sp.
Stereodivergent hydrodefluorination of gem-difluoroalkenes: selective synthesis of (Z)- and (E)-monofluoroalkenes.
We have developed a novel approach for the stereodivergent hydrodefluorination of gem-difluoroalkenes using copper(i) catalysts to obtain stereodefined monofluoroalkenes. Both (Z)- and (E)-terminal… Expand