Reference Genes for Accurate Transcript Normalization in Citrus Genotypes under Different Experimental Conditions
- V. Mafra, K. Kubo, M. Machado
- BiologyPLoS ONE
- 9 February 2012
Assessment of 15 candidate genes to determine which set of reference genes is best suited for transcript normalization in citrus in different tissues and organs and leaves challenged with five pathogens revealed FBOX, SAND, GAPC2 and UPL7 to be superior reference genes, and are recommended for use in studies of gene expression in citrus species and relatives.
Citrus Leprosis: Centennial of an Unusual Mite-Virus Pathosystem.
- M. Bastianel, V. M. Novelli, J. Freitas-AstĂșa
- Biology, MedicinePlant Disease
- 3 February 2010
Global gene expression of Poncirus trifoliata, Citrus sunki and their hybrids under infection of Phytophthora parasitica
- L. P. Boava, M. Cristofani-Yaly, M. Machado
- BiologyBMC Genomics
- 17 January 2011
The results indicated that the selected transcripts were probably involved in the whole process of plant defense responses to pathogen attack, including transcriptional regulation, signaling, activation of defense genes participating in HR, single dominant genes (R gene) such as TIR-NBS-LRR and RPS4 and switch of defense-related metabolism pathway.
Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae) transmitted virus
- E. Kitajima, K. Kubo, R. Salaroli
- Biology
- 1 February 2008
Exames preliminares de seccoes de tecido das manchas cloroticas ao microscopio eletronico revelaram a ocorrencia de efeitos citopaticos caracteristicos dos induzidos pelos virus do tipo nuclear, transmitido por acaros Brevipalpus (VTB).
Functional Annotation of Chemical Libraries across Diverse Biological Processes
- J. Piotrowski, Sheena C. Li, Charles Boone
- BiologybioRxiv
- 28 February 2017
A highly parallel and unbiased yeast chemical-genetic screening system involving three key components, which constructed an optimized, diagnostic mutant collection that is predictive all major yeast biological processes and predicted compound functionality.
Orchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus
- K. Kubo, J. Freitas-AstĂșa, M. A. Machado, E. Kitajima
- BiologyJournal of General Plant Pathology
- 8 May 2009
This virus was not amplified by the CiLV-C and SvRSV primers, and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they are definitely different viruses.
Yeast species-specific, differential inhibition of ÎČ-1,3-glucan synthesis by poacic acid and caspofungin
- Keunsook K Lee, K. Kubo, N. Gow
- BiologyThe Cell Surface
- 1 September 2018
Citrus leprosis virus C infects Arabidopsis thaliana, the model for plant-pathogen interactions
- G. Arena, M. Bergamini, A. Tassi, E. Kitajima, K. Kubo, J. Freitas-AstĂșa
- Biology
- 31 July 2013
Besides extending the range of CiLV-C hosts to a member of the family Brassicaceae, the use of A. thaliana in further studies will provide the opportunity for uncovering mechanisms involved in host-virus interaction.
Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid gene
- K. Kubo, R. M. Stuart, E. Kitajima
- BiologyArchives of Virology
- 21 May 2009
The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing, and none of the analyses showed geographic correlation among the Brazilian strains.
Profiling of the effects of antifungal agents on yeast cells based on morphometric analysis.
- Abraham A. Gebre, Hiroki Okada, Cholgwang Kim, K. Kubo, S. Ohnuki, Y. Ohya
- BiologyFEMS Yeast Research
- 1 August 2015
It is suggested that morphological profiling can be used to develop novel antifungal drugs and a systematic classifier that sorts a newly discovered compound into a class with a similar mode of action without any mutant information is developed.
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