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A tentative direct microscopic method for counting living marine bacteria.
Yeast extract and nalidixic acid were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark and the number of cells obtained is proposed as a direct cound of viable bacterial cells (DVC). Expand
A light-driven sodium ion pump in marine bacteria.
It is discovered that the marine flavobacterium Krokinobacter eikastus possesses two rhodopsins, the first, KR1, being a prototypical proton pump, while the second, KR2, pumps sodium ions outward. Expand
Role of sub-micrometre particles in the ocean
PARTICULATE matter plays an important part in biogeochemical cycles in the ocean; as particles settle out of the water column, they carry with them carbon and other adsorbed chemicals. BecauseExpand
Diversity and functional analysis of proteorhodopsin in marine Flavobacteria.
It is revealed that PR-mediated proton transport can create the sufficient membrane potential for the ATP synthesis in native flavobacterial cells. Expand
Distribution of viable marine bacteria in neritic seawater around Japan.
The direct viable court (DVC) method of determining the number of living bacteria in natural seawater was applied to samples collected from areas around Japan ranging from polluted to oligotrophic.Expand
Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria.
Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbon-degrading bacteria still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (Streptococcus and Staphylococcus) and bacillus ( Streptobacillus). Expand
A highly sensitive and specific multiplex PCR assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus
Aims:  To develop an effective multiplex PCR for simultaneous and rapid detection of Vibrio cholerae, Vibrio vulnificus and Vibrio parahaemolyticus, the three most important Vibrio species that canExpand
A global census of marine microbes
A tissue culture assay for tetrodotoxin, saxitoxin and related toxins.
In the presence of ouabain, veratridine enhances sodium influx in the mouse neuroblastoma cell line Neuro-2A (ATCC, CCL131), causing cellular swelling and subsequent death, and this phenomenon was used as the basis of a new assay for these toxins. Expand
Bacterial motility: links to the environment and a driving force for microbial physics.
While careful physical analysis has contributed to the current understanding of bacterial movement, tactic bacteria are increasingly widely used as experimental and theoretical model systems in physics. Expand