Cell-wall carbohydrates and their modification as a resource for biofuels.
Plant cell walls represent the most abundant renewable resource on this planet. Despite their great abundance, only 2% of this resource is currently used by humans. Hence, research into the…
Identification of Protein Transport Complexes in the Chloroplastic Envelope Membranes via Chemical Cross-Linking
It is proposed that each envelope membrane contains distinct translocation complexes and that a portion of these interact to form contact sites even in the absence of precursor proteins.
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component[W][OA]
It is concluded that XXT1 and XXT2 encode xylosyltransferases that are required for xyloglucan biosynthesis in vivo and this results in significant changes in the mechanical properties of these plants.
Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone
Stable interactions between precursor proteins and their associated membrane translocation components in detergent‐solubilized chloroplastic membrane fractions are identified and possible roles for stromal Hsp100 in protein import and mechanisms of precursor binding in chloroplasts are discussed.
Envelope membrane proteins that interact with chloroplastic precursor proteins.
It is proposed that prSSU first binds in an ATP-independent fashion to the 86-kD protein, and the energy-requiring step is association with the 75- kD protein and assembly of a translocation contact site between the inner and outer membrane of the chloroplastic envelope.
Tic40, a membrane‐anchored co‐chaperone homolog in the chloroplast protein translocon
It is proposed that Tic40 functions as a co‐chaperone in the stromal chaperone complex that facilitates protein translocation across the inner membrane.
Protein import into chloroplasts.
Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
The more than 13,400 plant ESTs that are currently available provide a new resource that will facilitate progress in many areas of plant biology and are illustrated by a description of the results obtained from analysis of 1500 Arabidopsis ESTs from a cDNA library prepared from equal portions of poly(A+) mRNA from etiolated seedlings, roots, leaves, and flowering inflorescence.
The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1
- G. Vanzin, M. Madson, N. Carpita, N. Raikhel, K. Keegstra, W. Reiter
- Biology, Environmental ScienceProceedings of the National Academy of Sciences…
- 19 February 2002
The normal growth habit and wall strength of mur2 plants casts doubt on hypotheses regarding roles of xyloglucan fucosylation in facilitating xylglucan–cellulose interactions or in modulating growth regulator activity.
Protein Import and Routing Systems of Chloroplasts
Plastids are ubiquitous plant cell organelles that perform many essential functions. Chloroplasts are the most complex type of plastid, both structurally and functionally, containing six distinct…