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Phosphorylation and Activation of Myosin by Rho-associated Kinase (Rho-kinase)*
  • M. Amano, Masaaki Ito, +5 authors K. Kaibuchi
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 23 August 1996
The phosphorylation of MLC by Rho-kinase resulted in the facilitation of the actin activation of myosin ATPase, which may partly account for the mechanism by which Rho regulates cytokinesis, cell motility, or smooth muscle contraction. Expand
Regulation of Myosin Phosphatase by Rho and Rho-Associated Kinase (Rho-Kinase)
Rho appears to inhibit myosin phosphatase through the action of Rho-kinase, which is activated by GTP·RhoA, phosphorylation of MBS and MLC in NIH 3T3 cells. Expand
Small GTP-binding proteins.
In this review, functions of small G proteins and their modes of activation and action are described. Expand
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
Kinetic analysis indicates that TPA can substitute for diacylglycerol and greatly increases the affinity of the enzyme for Ca2+ as well as for phospholipid, and various phorbol derivatives which have been shown to be active in tumor promotion are also capable of activating this protein kinase in in vitro systems. Expand
Rho-Kinase Phosphorylates COOH-terminal Threonines of Ezrin/Radixin/Moesin (ERM) Proteins and Regulates Their Head-to-Tail Association
Observations indicate that the Rho-kinase–dependent phosphorylation interferes with the intramolecular and/ or intermolecular head-to-tail association of ERM proteins, which is an important mechanism of regulation of their activity as actin filament/plasma membrane cross-linkers. Expand
GSK-3β Regulates Phosphorylation of CRMP-2 and Neuronal Polarity
The results suggest that NT-3 decreases phosphorylated CRMP-2 and increases nonphosphorylated active CR MP-2, thereby promoting axon outgrowth, and GSK-3beta regulates neuronal polarity through the phosphorylation of CRMC-2. Expand
Formation of Actin Stress Fibers and Focal Adhesions Enhanced by Rho-Kinase
Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions. Expand
Rac1 and Cdc42 Capture Microtubules through IQGAP1 and CLIP-170
Results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization. Expand
Rho‐associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho.
P purified a Rho‐interacting protein with a molecular mass of approximately 164 kDa (p164) from bovine brain that bound to GTPgammaS (a non‐hydrolyzable GTP analog) and is likely to be a putative target serine/threonine kinase for Rho and serves as a mediator of the RHo‐dependent signaling pathway. Expand
Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells.
The role of the Rho-Rho-kinase pathway in contractile processes of smooth muscle and in non-muscle cell functions, and the pathophysiological implications of this pathway are summarized. Expand