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Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii.
The assay was useful to identify cultures after in vitro cultivation and to detect and identify A. butlzeri and A. cryaerophilus from poultry samples present in 24-h old enrichment in Arcobacter broth with cefoperazone, amphotericin and teicoplanin (CAT)-supplement. Expand
Isolation of Arcobacter species from animal feces.
Good repeatability, in-lab reproducibility and sensitivity were achieved and the specificity was improved by additional incorporation of cycloheximide and increase of the novobiocin concentration in the selective supplement. Expand
Prevalence, enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium.
Characterization of the 164 isolates showed a large heterogeneity and animals could be colonized with more than one genotype, but Arcobacter cryaerophilus was the dominant species isolated from cows, but co-colonizations occurred in 26% of theArcobacter excreting animals. Expand
Arcobacter cibarius sp. nov., isolated from broiler carcasses.
Data demonstrate that the 20 isolates represent a single novel Arcobacter species, for which the nameArcobacter cibarius sp. Expand
Assessment of the Genetic Diversity among Arcobacters Isolated from Poultry Products by Using Two PCR-Based Typing Methods
Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and randomly amplified polymorphic DNA PCR (RAPD- PCR) were optimized for characterization of Arcobacter butzleri, Arcobacteria cryaerophilus, and Arcob bacteria skirrowii, demonstrating that different outcomes can be obtained in epidemiological studies depending on the isolation procedure used and the number of isolates characterized. Expand
Isolation and characterization of the emerging foodborn pathogen Arcobacter from human stool.
  • K. Houf, R. Stephan
  • Biology, Medicine
  • Journal of microbiological methods
  • 1 February 2007
Though the pathogenic role and potential virulence factors of arcobacters have to be further examined, the current status of Arcobacter as emerging pathogens remains justified. Expand
Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products.
The prevalence of Arcobacter in Belgian poultry was found higher than the prevalence of thermophilic Campylobacter species in each of the poultry categories examined. Expand
Occurrence and strain diversity of Arcobacter species isolated from healthy Belgian pigs.
Large heterogeneity among the Arcobacter species was detected in pigs and on the farms, and simultaneous shedding of two or three species occurred. Expand
Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Humans and Animals
The presence of nine putative Campylobacter virulence genes previously identified in the recent Arcobacter butzleri ATCC 49616 genome sequence was determined in a large set of human and animal Arcobacterial strains after the development of rapid and accurate PCR assays and confirmed by sequencing and dot blot hybridization. Expand
Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium.
Fluoroquinolones should be considered in the treatment of severe C. upsaliensis and A. butzleri infection and when clinically indicated, erythromycin remains the first choice for the Treatment of intestinal campylobacteriosis caused by C. concisus and C. fetus. Expand