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Mixed chimerism following in utero hematopoietic stem cell transplantation in murine models of hemoglobinopathy.
OBJECTIVE Mixed hematopoietic chimerism after bone marrow transplantation can provide effective treatment for beta-thalassemia because of the selective advantage that exists for donor erythropoiesis.Expand
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Flexibility and nucleation in sickle hemoglobin.
We have studied the self-assembly of Hemoglobin C-Harlem (HbC-Harlem), a double mutant of hemoglobin that possesses the beta6 Glu-->Val mutation of sickle hemoglobin (HbS) plus beta73 Asp-->Asn. ByExpand
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Combined use of nonmyelosuppressive nitrosourea analogues with hydroxyurea in the induction of F-cell production in a human erythroleukemic cell line.
OBJECTIVE Although hydroxyurea (HU) has been used clinically to treat patients with sickle cell disease (SCD), not all patients benefit from HU treatment due to its toxicity. The objective of thisExpand
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Enhancement of hemoglobin and F‐cell production by targeting growth inhibition and differentiation of K562 cells with ribonucleotide reductase inhibitors (didox and trimidox) in combination with
Upon appropriate drug treatment, the human erythroleukemic K562 cells have been shown to produce hemoglobin and F‐cells. Fetal hemoglobin (Hb F) inhibits the polymerization events of sickleExpand
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Effects of different beta73 amino acids on formation of 14-stranded fibers of Hb S versus double-stranded crystals of Hb C-Harlem.
Hb S (alpha(2)beta(2)(6Glu-->Val)) forms polymers, while Hb C-Harlem (alpha(2)beta(2)(6Glu-->Val,73Asp-->Asn)) forms crystals upon oversaturation. Since the only difference between the two is theExpand
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Oxygen Promotes Sickling of SS Cells a
The effect of oxygen on the formation of sickled cells was studied. Previously we found that the morphology of sickle cells (SS cells) under deoxygenated conditions depends highly on the method ofExpand
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The mutH gene regulates the replication and methylation of the pMB1 origin.
DNA methylation is known to regulate several prokaryotic replication origins. In particular, the Escherichia coli chromosomal origin oriC and the pMB1 plasmid origin (which is homologous to the ColE1Expand
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