UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development
It is shown that the human JmjC-domain-containing proteins UTX and JMJD3 demethylate tri-methylated Lys 27 on histone H3, and the results suggest that H3K27me3 dem methylation regulated by UTX/JMJD3 proteins is essential for proper development.
Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their…
TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity
It is proposed that TET1 fine-tunes transcription, opposes aberrant DNA methylation at CpG-rich sequences and thereby contributes to the regulation ofDNA methylation fidelity.
EZH2 is downstream of the pRB‐E2F pathway, essential for proliferation and amplified in cancer
- A. Bracken, D. Pasini, M. Capra, E. Prosperini, E. Colli, K. Helin
- BiologyEMBO Journal
- 15 October 2003
It is demonstrated that both EZH2 and EED are essential for the proliferation of both transformed and non‐transformed human cells and, in addition, the pRB‐E2F pathway tightly regulates their expression and, consistent with this, it is found that EZh2 is highly expressed in a large set of human tumors.
Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity
It is shown that mice lacking Suz12 are not viable and die during early postimplantation stages displaying severe developmental and proliferative defects, and an essential role of SUZ12 in regulating the activity of the PRC2/3 complexes, which are required for regulating proliferation and embryogenesis.
The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation
- D. Pasini, A. Bracken, J. Hansen, M. Capillo, K. Helin
- BiologyMolecular and Cellular Biology
- 5 March 2007
It is demonstrated that Suz12 is required for the establishment of specific expression programs required for ES cell differentiation and evidence that PcGs have different mechanisms to regulate transcription during cellular differentiation is provided.
The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3
In addition to identifying GASC1 as a histone trimethyl demethylase, this work suggests a model for how this enzyme might be involved in cancer development, and proposes it as a target for anti-cancer therapy.
The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells.
The ability of the oncogene BMI1 to repress the INK4A-ARF locus requires its direct association and is dependent on the continued presence of the EZH2-containing Polycomb-Repressive Complex 2 (PRC2) complex.
Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein.
It is shown that, like pRB, p107 is a potent inhibitor of E2F-mediated trans-activation, and overexpression of p107 can inhibit proliferation in certain cell types, arresting sensitive cells in G1.
E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis.
It is shown that the E2Fs control the expression of several genes that are involved in cell proliferation and apoptosis, differentiation, and development and provide possible genetic explanations to the variety of phenotypes observed as a consequence of a deregulated pRB/E2F pathway.