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Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy
- Yongtao Zhu, Lanlan Han, K. Hefferon, N. Silvaggi, D. Wilson, M. McBride
- BiologyApplied and Environmental Microbiology
- 3 June 2016
The results suggest that both cell surface and periplasmic endoglucanases are required for the growth of C. hutchinsonii on cellulose and that novel cell surface proteins may solubilize and transport cellodextrins across the outer membrane.
Multi‐tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation
While both Rep and RepA are found to behave as equally potent inhibitors of complementary‐sense gene expression, they differ considerably with respect to their abilities to transactivate virion‐ sense gene expression.
Host cell receptor binding by baculovirus GP64 and kinetics of virion entry.
Specific competition by GP64solOp for virion binding suggests that the GP64 protein is a host cell receptor-binding protein.
Nutritionally Enhanced Food Crops; Progress and Perspectives
- K. Hefferon
- BiologyInternational journal of molecular sciences
- 1 February 2015
The improvement of the nutritional status of crops to make a positive impact on global human health is discussed and examples of nutritionally enhanced crops which have been developed using biotechnological approaches will be discussed.
Plant Virus Expression Vector Development: New Perspectives
- K. Hefferon
- BiologyBioMed research international
- 13 March 2014
This review presents recent innovations in plant virus expression systems and their uses for producing biologics from plants.
Expression of a vaccine protein in a plant cell line using a geminivirus-based replicon system.
Comparative analysis of species-independent, isozyme-specific amino-acid substitutions in mammalian muscle, brain and liver glycogen phosphorylases.
Potexvirus isolation and RNA extraction.
Independent expression of Rep and RepA and their roles in regulating bean yellow dwarf virus replication.
Constitutive expression of both Rep and RepA supported replication and enhanced gene expression and the effect of a RepA mutant with an altered retinoblastoma-related-protein binding motif on the efficiency of BeYDV replication was found to severely diminish replication efficiency.
Expression of the coat protein of potato virus X from a dicistronic mRNA in transgenic potato plants.
- K. Hefferon, H. Khalilian, H. Xu, M. Abouhaidar
- BiologyThe Journal of general virology
- 1 November 1997
Results suggest that potexvirus CP expression may take place from the genomic RNA as well as from the subgenomic RNA.