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Novel Genes Encoding 2-Aminophenol 1,6-Dioxygenase fromPseudomonas Species AP-3 Growing on 2-Aminophenol and Catalytic Properties of the Purified Enzyme*
2-Aminophenol 1,6-dioxygenase was purified from the cell extracts of Pseudomonas sp. AP-3 grown on 2-aminophenol. The product from 2-aminophenol by catalysis of the purified enzyme was identified as
Molecular Analysis of Maleate cis-trans Isomerase from Thermophilic Bacteria
TLDR
The results suggest that Cys80 and Cys198 play important roles in the enzyme activity of thermophilic bacteria, and are replaced with serine by site-directed mutagenesis.
Genomic organization of the biotin biosynthetic genes of coryneform bacteria: cloning and sequencing of the bioA-bioD genes from Brevibacterium flavum.
TLDR
The cloning and sequencing of the biotin biosynthetic genes encoding the 7,8-diaminopelargonic acid aminotransferase and the dethiobiotin synthetase of B. flavum were reported and the genomic organization of the bioA, bioB and bioD genes in B. Flavum has been shown to be different from that in E. coli and B. sphaericus.
Replacement recombination in Coryneform bacteria: High efficiency integration requirement for non-methylated plasmid DNA
We have developed a versatile system that permits efficient manipulation of coryneform bacteria by gene disruption and replacement. Circumventing the methyl-specific restriction system in certain of
Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum.
TLDR
Three strains of coryneform bacteria tested were able to convert 7-keto-8-aminopelargonic acid to biotin, through a biotin synthetic pathway identical to that from E. coli, indicating that the bioB gene product is necessary for the conversion of dethiobiotin toBiotin.
Cloning, Sequencing, and Characterization of theftsZGene from Coryneform Bacteria
TLDR
Taking advantage of highly conserved domains present in the ftsZ genes from Escherichia coli, Rhizobium meliloti, and Bacillus subtilis, degenerate oligonucleotides corresponding to these regions were designed and used as primers in PCR in order to amplify DNA sequences from Brevibacterium flavum MJ233 chromosomal DNA.
Gene cloning and characterization of maleate cis-trans isomerase from Alcaligenes faecalis.
TLDR
Maleate cis-trans isomerase, which catalyses the conversion of maleate to fumarate, was purified and characterized from Alcaligenes faecalis IFO13111 and shows no significant homology to any amino acid sequences in the protein data base.
Analysis of biotin biosynthesis pathway in coryneform bacteria: Brevibacterium flavum.
TLDR
The chapter reveals the deleted steps of the biotin biosynthetic pathway in several coryneform bacteria by using cross-feeding experiments with E. coil bio mutants.
Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria.
TLDR
Taking advantage of highly conserved domains present in the ftsZ genes from Escherichia coli, Rhizobium meliloti, and Bacillus subtilis, degenerate oligonucleotides corresponding to these regions were designed and used as primers in PCR in order to amplify DNA sequences from Brevibacterium flavum MJ233 chromosomal DNA.
Electrotransformation of intact cells ofBrevibacterium flavum MJ-233
TLDR
Electroporation allowed transformation of intact cells of Brevibacterium flavum flavum MJ-233, and the efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was imporved by the addition of 1.0U/ml of penicillin G to the culture medium.
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