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Functional role of the MrpA- and MrpD-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex I.
The corresponding antiporter-like protein subunits from the smaller enzymes evolutionarily related to complex I were tested and behaved essentially as those from full size complex I, corroborating that this enzyme should be regarded as a bona fide complex I. Expand
Electrophoretic deposition of organic/inorganic composite coatings containing ZnO nanoparticles exhibiting antibacterial properties.
To address one of the serious problems associated with permanent implants, namely bacterial infections, novel organic/inorganic coatings containing zinc oxide nanoparticles (nZnO) are proposed.Expand
Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes.
The pH-sensitive fluorescent nanoprobe Glu(3), which is well suited for pH measurements in microcompartmentalized biological systems, was found to be completely membrane impermeable, and its distinct spectroscopic features permit pH measurements inside closed membrane vesicles, enabling quantitative mechanistic studies of membrane-spanning proteins. Expand
Structure and function of the C‐terminal domain of MrpA in the Bacillus subtilis Mrp‐antiporter complex – The evolutionary progenitor of the long horizontal helix in complex I
The C‐terminal domain of MrpA, outside this conserved area, was investigated and the transmembrane orientation was found to correspond to that of NuoJ in complex I. Expand
Cu-Mg-Fe-O-(Ce) complex oxides as catalysts of selective catalytic oxidation of ammonia to dinitrogen (NH3-SCO)
Multicomponent oxide systems 800-Cu-Mg-Fe-O and 800-Cu-Mg-Fe-O-Ce were tested as catalysts of selective catalytic oxidation of ammonia to dinitrogen (NH3-SCO) process. Materials were obtained byExpand
Electrochemical communication between heterotrophically grown Rhodobacter capsulatus with electrodes mediated by an osmium redox polymer.
The metabolically versatile purple bacteria Rhodobacter capsulatus was investigated to check its possible applicability in biofuel cells and electrochemical microbial biosensors, and demonstrates that development of photosensors and other light driven bioelectrochemical devices could be feasible using R. Capsulatus. Expand
Electrochemical Communication Between Electrodes and Rhodobacter capsulatus Grown in Different Metabolic Modes
The majority of efforts on microbial and photosynthetic microbial fuel cells are both curiosity driven and made to possibly meet the future growing demand for sustainable energy. The mostExpand
A cytochrome c fusion protein domain for convenient detection, quantification, and enhanced production of membrane proteins in Escherichia coli—Expression and characterization of cytochrome‐tagged
Overproduction of membrane proteins can be a cumbersome task, particularly if high yields are desirable. NADH:quinone oxidoreductase (Complex I) contains several very large membrane‐spanning proteinExpand
Cisplatin-induced elongation of Shewanella oneidensis MR-1 cells improves microbe–electrode interactions for use in microbial fuel cells
Cells of a known exoelectrogen, Shewanella oneidensis MR-1, were treated with cisplatin, a drug that inhibits cell division but forces the cells to grow longer resulting in a characteristic elongated growth, showing a 5-fold improvement in current densities. Expand
The Na+ transport in gram-positive bacteria defect in the Mrp antiporter complex measured with 23Na nuclear magnetic resonance.
The sodium-sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted could indeed be correlated to the inability of this strain to maintain a lower internal Na(+) concentration than an external one. Expand