• Publications
  • Influence
Plasmid copy number and plasmid stability.
  • K. Friehs
  • Medicine, Biology
  • Advances in biochemical engineering/biotechnology
  • 2004
Many expression systems in research and industry use plasmids as vectors for the production of recombinant proteins or non-proteinous recombinant substances. Plasmids have an essential impact onExpand
  • 194
  • 8
Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization
BackgroundThe classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although thisExpand
  • 42
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Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion.
In this study the use of Euglena gracilis biomass for α-tocopherol, paramylon and biogas production in a value-added chain was investigated. Therefore, we analyzed the dry cell weight and productExpand
  • 27
  • 4
An in situ probe for on‐line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning
Fermentation industries would benefit from on‐line monitoring of important parameters describing cell growth such as cell density and viability during fermentation processes. For this purpose, an inExpand
  • 43
  • 3
Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors
Abstract. The gene for phytase from Escherichia coli was sequenced and compared with the appA gene. It was found to be a mutant derivative of the appA gene. After fusion with a C-terminal His-tag,Expand
  • 49
  • 3
Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions
Usually, the fatty acid (FA) composition of lipids from microalgae is determined using samples taken at a single time point only, often without considering the medium composition and cultivationExpand
  • 20
  • 3
The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12.
The use of Escherichia coli as a model organism has provided a great deal of basic information in biomolecular sciences. Examining trait differences among closely related strains of the same speciesExpand
  • 29
  • 3
Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins.
Aiming to facilitate the accessibility of recombinant proteins produced with Escherichia coli, extracellular expression may be achieved by means of bacteriocin release protein (BRP) coexpression.Expand
  • 22
  • 3
A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase
Abstract For the feeding-phase of a batch/fed-batch cultivation of a recombinant Escherichia coli producing extracellular phytase, a controller has been developed. Based on the estimated processExpand
  • 32
  • 3
Extracellular production of a hybrid β-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors
Abstract Cultivation conditions for the extracellular production of a hybrid β-glucanase from Bacillus were established by using Escherichiacoli JM109 carrying the plasmid pLF3. This plasmidExpand
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