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Application of metal coordination chemistry to explore and manipulate cell biology.
Inorganic compounds should not be overlooked in the realm of chemical biology, since their distinctive electronic, chemical, and photophysical properties render them particularly useful for a variety of applications.
A Mets motif peptide found in copper transport proteins selectively binds Cu(I) with methionine-only coordination.
It is established that a relatively simple peptide containing an MX(2)MX( 2)M motif is sufficient to bind Cu(I) with an affinity that corresponds well with its proposed biological function of extracellular copper acquisition.
Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings.
A protein fusion construct of human ubiquitin with an N-terminal lanthanide binding tag (LBT) enables observation of long-range orientational restraints in solution NMR from residual dipolar
Lanthanide‐Binding Tags as Versatile Protein Coexpression Probes
This work presents peptide sequences with a 40‐fold higher affinity for Tb3+ ions and significantly brighter luminescence intensity compared with existing peptides, and introduces the LBT strategy, a new alternative for expressing fluorescent fusion proteins by routine molecular biological techniques.
Model peptides provide new insights into the role of histidine residues as potential ligands in human cellular copper acquisition via Ctr1.
Results from live cell studies support the hypothesis that extracellular amino-terminal His residues directly participate in the copper transport function of Ctr1, and suggest that the histidine domains may play a direct role in copper acquisition from serum copper-binding proteins and in facilitating the reduction of Cu(II) to the active Ctr 1 substrate, Cu(I).
Fe(III)-coordination properties of neuromelanin components: 5,6-dihydroxyindole and 5,6-dihydroxyindole-2-carboxylic acid.
Oxidation studies reveal that iron accelerates the air oxidation of DHI and DHICA, which are stronger Fe(III) chelates than catechol, dopamine, and Me-DHI at pH values from 3 to 10.
A Powerful Combinatorial Screen to Identify High‐Affinity Terbium(III)‐Binding Peptides
A new screening methodology is presented for the identification of new LBT sequences with high affinity for Tb3+ ions and intense luminescence properties that demonstrated a 140‐fold increase in affinity over previously reported lanthanide‐binding peptides.