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RNA-Guided Human Genome Engineering via Cas9
Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targetedExpand
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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes. Here we engineer this system to enable RNA-guided genome regulation inExpand
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Heritable genome editing in C. elegans via a CRISPR-Cas9 system
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line usingExpand
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Cas9 as a versatile tool for engineering biology
RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the genomes of diverseExpand
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Concerning RNA-guided gene drives for the alteration of wild populations
Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here weExpand
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Concerning RNA-Guided Gene Drives for the Alteration of Wild Populations
Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here weExpand
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Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing
The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas acquired immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but it canExpand
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Safeguarding CRISPR-Cas9 gene drives in yeast
RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, theExpand
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A System for the Continuous Directed Evolution of Biomolecules
Laboratory evolution has generated many biomolecules with desired properties, but a single round of mutation, gene expression, screening or selection, and replication typically requires days orExpand
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Daisy-chain gene drives for the alteration of local populations
Significance CRISPR-based gene drive systems—genetic elements which could be engineered to rapidly spread traits through wild populations—could help solve some of humanity’s greatest ecological andExpand
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