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CombiMatrix oligonucleotide arrays: genotyping and gene expression assays employing electrochemical detection.
TLDR
The ElectraSense platform has been used to develop nucleic acid assays for highly accurate genotyping of a variety of pathogens including bio-threat agents and common pathogens of the respiratory tract. Expand
Detection of salmonella in poultry using a silicon chip-based biosensor.
TLDR
An experiment whereby chicken carcass washings were fortified with salmonella showed a recovery of 90%, indicating that the Threshold Immunoassay System can be used to test forSalmonella under these conditions. Expand
Natural-abundance, 13C-nuclear magnetic resonance-spectral studies of carbohydrates linked to amino acids and proteins.
TLDR
This chapter presents the 13 C-nuclear magnetic resonance-spectral studies of carbohydrates linked to amino acids and proteins in terms of the information that may be gained about the glycoprotein without its modification. Expand
Determination of dissociation constant and concentration of an anti-DNA antibody by using the light-addressable potentiometric sensor.
TLDR
The most sensitive assay possible is desired for quantitation of total DNA and DNA probe binding, which requires the use of a specific antibody with a high affinity for DNA. Expand
Immunoassays based on electrochemical detection using microelectrode arrays.
TLDR
The results for human alpha1 acid glycoprotein, ricin, M13 phage, Bacillus globigii spores, and fluorescein indicate that this method is one of the most sensitive available, with limits of detection in the attomole range. Expand
Self-assembling protein arrays using electronic semiconductor microchips and in vitro translation.
TLDR
A model two-protein microarray is demonstrated using luciferase and green fluorescent protein, both expressed in a cell-free system and specifically immobilized on CombiMatrix semiconductor oligonucleotide microarrays, providing evidence for the appropriate folding, activity, robust presentation, and efficient flexible detection of proteins on the microscale. Expand
Specific 13C reductive methylation of glycophorin A. Possible relation of the N-terminal amino acid and the lysine residues to MN blood group specificities.
TLDR
Heterozygous and homozygous glycophorin A were partially and fully reductively methylated with 13C-enriched formaldehyde in the presence of sodium cyanoborohydride, suggesting that for the N alpha,N-[13C]dimethyl Ser derivative of glycoph orin AM, hindered rotation must occur about one of the Nalpha-13CH3 bonds. Expand
Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
TLDR
An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. Expand
Detection of plasmids using DNA and RNA probes and the light-addressable potentiometric sensor.
TLDR
Intact plasmids, plasmid fragments, and cDNA were detected using two DNA or RNA probes of varying lengths, each containing only biotin or fluorescein molecules using the light-addressable potentiometric sensor. Expand
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