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Evolution of catalytic and regulatory sites in phosphorylases
The sequence of 687 amino acids of Escherichia coli maltodextrin phosphorylase, deduced from a cloned malP gene sequence is reported, concluding that the regulatory sequences that also carry the phosphorylation site in the muscle enzyme were joined to a presumed ancestral precursor gene by gene fusion after separation of the eukaryotic and prokaryotic lines of descent.
Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.
Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134 and this technique was used to identify cistrons ofSPP1 involved in phage DNA synthesis and to define intermediates in SPP 1 replication.
Fungicidal effect of tyrothricin on Candida albicans
Because fungicidal concentrations of tyrothricin are locally achievable in patients, an evaluation of the local effect of ty Rothricin in patients suffering from mucosal infections with Candida species should be considered.
Specific labelling of replicating SPP1 DNA
SummarySpecific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under…
Purification and characterization of DNA polymerase from the archaebacterium Methanobacterium thermoautotrophicum
Purification and characterization of DNA polymerase from the archaebacterium Sulfolobus acidocaldarius.
DNA polymerase has been purified about 25,000-fold from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius and characterized in respect to optimal assay conditions, template specificity, sensitivity to inhibitors and associated nuclease activities.
A site-specific endonuclease activity in Halobacterium halobium
In Halobacterium halobium and some related strains, a site-specific endonuclease activity was found and the 3 cleavage sites in plasmid pBR322 were mapped, but no homology between these sites was found.
Sensitivity of halobacteria to aphidicolin, an inhibitor of eukaryotic α-type DNA polymerases
From analyses of macromolecular syntheses in aphidicolin-treated halobacteria, it is concluded that the primary target of this drug is DNA synthesis, possibly a DNA polymerase, which implies an evolutionary relationship to eukaryotic DNA replication.
Expression and Instability of Heterologous Genes in B. subtilis
Prophage induction by alkylating ethyl methylaminosulfonate.
Treatment of the lysogenic strain M. lysodeikticus 53-40 (N5) with ethyl methylaminosulfonate results in a slight increase in infective center concentration of N5 phages which is only detectable once…