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Molecular architecture of a kinetochore–microtubule attachment site
Kinetochore attachment to spindle microtubule plus-ends is necessary for accurate chromosome segregation during cell division in all eukaryotes. The centromeric DNA of each chromosome is linked toExpand
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Budding Yeast Chromosome Structure and Dynamics during Mitosis
Using green fluorescent protein probes and rapid acquisition of high-resolution fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle polesExpand
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The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae
Microtubule assembly in Saccharomyces cerevisiae is initiated from sites within spindle pole bodies (SPBs) in the nuclear envelope. Microtubule plus ends are thought to be organized distal to theExpand
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Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome
Quantitative measurement of the number of Cse4, CBF3, and Ndc80 proteins at kinetochores reveals a 2.5–3-fold increased copy number relative to prior estimates.
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In Vivo Protein Architecture of the Eukaryotic Kinetochore with Nanometer Scale Accuracy
The kinetochore is a macromolecular protein machine [1] that links centromeric chromatin to the plus ends of one or more microtubules (MTs) and segregates chromosomes during cell division. Its coreExpand
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The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast.
The budding yeast kinetochore is comprised of >60 proteins and associates with 120 bp of centromeric (CEN) DNA. Kinetochore proteins are highly dynamic and exhibit programmed cell cycle changes inExpand
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Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular minichromosomes
We have examined the chromatin structure of the centromere regions of chromosomes III and XI in yeast by using cloned functional centromere DNAs (CEN3 and CEN11) as labeled probes. When chromatinExpand
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Pericentric Chromatin Is an Elastic Component of the Mitotic Spindle
BACKGROUND Prior to chromosome segregation, the mitotic spindle bi-orients and aligns sister chromatids along the metaphase plate. During metaphase, spindle length remains constant, which suggestsExpand
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Pericentric Chromatin Is Organized into an Intramolecular Loop in Mitosis
BACKGROUND Cohesin proteins link sister chromatids and provide the basis for tension between bioriented sister chomatids in mitosis. Cohesin is concentrated at the centromere region of the chromosomeExpand
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Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres
Point and regional centromeres specify a unique site on each chromosome for kinetochore assembly. The point centromere in budding yeast is a unique 150-bp DNA sequence, which supports a kinetochoreExpand
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