Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
- K. Asada
- Environmental Science, EngineeringAnnual Review of Plant Physiology and Plant…
- 1 June 1999
Whenever the water-water cycle operates properly for scavenging of active oxygens in chloroplasts, it also effectively dissipates excess excitation energy under environmental stress.
Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions1
- K. Asada
- Environmental SciencePlant Physiology
- 1 June 2006
The reaction centers of PSI and PSII in chloroplast thylakoids are the major generation site of reactive oxygen species (ROS) and the primary reduced product was identified.
Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants
- K. Asada
- 1 June 1992
Amino acid sequence and other molecular properties indicate that ascorbate peroxidase resembles cytochrome c peroxIDase from fungi rather than guaiacol per oxidase from plants, and it is proposed that the plant and yeast hydrogen peroxide-scavenging peroxidsases have the same ancestor.
Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical
Monodehydroascorbate Reductase in Spinach Chloroplasts and Its Participation in Regeneration of Ascorbate for Scavenging Hydrogen Peroxide
Ascorbate Peroxidase in Tea Leaves: Occurrence of Two Isozymes and the Differences in Their Enzymatic and Molecular Properties
Two isozymes of ascorbate (AsA) peroxidase were found in tea leaves, and one of them was purified to homogeneity, as judged by polyacrylamide gel electrophoresis.
Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts and Photoreduction of Its Primary Oxidation Product Monodehydroascorbate Radicals in Thylakoids
Ascorbate is photoregenerated in the thylakoids from the MDA radicals produced in a reaction of asCorbate peroxidase for the scavenging of hydrogen peroxide.