• Publications
  • Influence
Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.
TLDR
It is concluded that bafilomycin A1 is a valuable tool for distinguishing among the three different types of ATPases and represents the first relatively specific potent inhibitor of vacuolar ATPases. Expand
Bafilomycins and concanamycins as inhibitors of V-ATPases and P-ATPases.
TLDR
Two groups of the plecomacrolide-defined class of macrolide antibiotics are recognized as important tools for studying the physiological role of vacuolar-type, proton-translocating ATPases and ATPases with phosphorylated states in animal and plant cells as well as in yeast, fungi and bacteria. Expand
Inhibitory effect of modified bafilomycins and concanamycins on P- and V-type adenosinetriphosphatases.
TLDR
The structure-activity study showed that in general the concanamycins, 18-membered macrolides, are better and more specific inhibitors than the bafilomycins of this class of membrane-bound ATPases. Expand
Concanamycin A, the Specific Inhibitor of V-ATPases, Binds to the Vo Subunit c*
TLDR
Binding of J-concanolide A to subunit c was prevented in a concentration-dependent manner by concanamycin A, indicating that labeling was specific, and binding was also prevented by the plecomacrolides bafilomycin A1 and B1, respectively. Expand
Osmotic Stress.
TLDR
Research frontiers include the osmoadaptive remodeling of cell structure, the mechanism by which osmotic stress alters gene expression, the mechanisms by which transporters and channels detect and respond to osmosis pressure changes, the coordination of osmoregulatory programs and selection of available osmoprotectants, and the roles played by osmoreGulatory mechanisms as E. coli and Salmonella survive or thrive in their natural environments. Expand
K+ and Ionic Strength Directly Influence the Autophosphorylation Activity of the Putative Turgor Sensor KdpD ofEscherichia coli *
TLDR
It is proposed that changes of intracellular parameters elicited by K+ limitation or osmotic upshock directly influence KdpD autophosphorylation activity, whereby K+ has an inhibitory and ionic strength a stimulatory effect. Expand
The F0F1-type ATP synthases of bacteria: structure and function of the F0 complex.
TLDR
Membrane-bound ATP synthases of bacteria serve two important physiological functions: the enzyme catalyzes the synthesis of ATP from ADP and inorganic phosphate utilizing the energy of an electrochemical ion gradient and generating a transmembrane ion gradient at the expense of ATP hydrolysis. Expand
KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators
TLDR
This mechanism is proposed to control expression of Kdp, a transport ATPase with high affinity for potassium, that is expressed when turgor pressure is low and is members of the sensor-effector class of regulatory proteins. Expand
Towards an understanding of the molecular mechanisms of stimulus perception and signal transduction by the KdpD/KdpE system of Escherichia coli.
TLDR
Structural features of KdpD and KdpE, which are important for stimulus perception and/or signal transduction were identified and are described here, and various studies undertaken to elucidate the nature of the stimulus result in a new model for KDPD stimulus perception. Expand
The KdpF Subunit Is Part of the K+-translocating Kdp Complex of Escherichia coli and Is Responsible for Stabilization of the Complex in Vitro *
TLDR
Upon expression of this operon in minicells, a so far unrecognized small hydrophobic polypeptide, KdpF, could be identified on high resolution SDS-polyacrylamide gels and proved to be indispensable for a functional enzyme complex in vitro. Expand
...
1
2
3
4
5
...