K. S. Shashidhara

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The conformational transitions in an oligomeric and high molecular weight class II α-mannosidase from Aspergillus fischeri were examined using fluorescence and CD spectroscopy under chemical, thermal and acid denaturing conditions. The enzyme lost the activity first and then the overall folded conformation and secondary structure. The midpoint values of(More)
Energetics of the catalysis of Class II alpha-mannosidase (E.C. from Aspergillus fischeri was studied. The enzyme showed Kcat/Km for Man (alpha1-3) Man, Man (alpha1-2) Man and Man (alpha1-6) Man as 7488, 5376 and 3690 M(-1) min(-1), respectively. The activation energy, Ea was 15.14, 47.43 and 71.21 kJ/mol for alpha1-3, alpha1-2 and alpha1-6 linked(More)
Apart from the vital role in glycoprotein biosynthesis and degradation, α-mannosidase is currently an important therapeutic target for the development of anticancer agents. Fluorescence quenching and time-resolved fluorescence of α-mannosidase, a multitryptophan protein from Aspergillus fischeri were carried out to investigate the tryptophan environment.(More)
A new series of heterocyclic amides were synthesized by the reaction of heterocyclic amines with substituted phenylacetic acids 1 in the presence of EDC.HCl as a coupling agent. The newly synthesized compounds were characterized by IR, NMR, mass spectral, and elemental analysis. All the synthesized compounds were evaluated for their in vivo analgesic,(More)
Various polyhydroxy piperidine azasugars have been synthesized from precursors 18a and 18b, obtained in both enantiomeric forms from d-ribose. Out of these polyhydroxy piperidine azasugars, 22, 39 and 20 were found to be potent as well as selective inhibitors of alpha-glucosidase with K(i) values ranging as low as 1.07 microM, 16.4 microM, and 88.2 microM,(More)
This paper discusses a rail to rail swing, novel CMOS transmission gate architecture based 1-bit 30-transistor full adder. Worst-case: power, delay and power delay product of this 1-bit full adder is compared with other two high performance 1-bit full adder architectures reported till date, at 90 nm technology node. The proposed 1-bit adder has 13.9%(More)
A highly divergent route to a variety of quinolizidine alkaloids is described. The enantiomeric precursors and utilized for the synthesis of these alkaloids were constructed stereospecifically from the PET cyclization of the corresponding acetylene tethered alpha-trimethylsilyl amine moieties and , respectively, both of which were synthesised from D-ribose.(More)
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