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This study describes three methods of computerized three-dimensional reconstruction of the main neurovascular pulpal bundle of human teeth, using serial cross-paraffin sections, digital image processing, and three-dimensional computer graphics. These methods are surface representation, representation by means of a stack of slices, and volume representation.
Five healthy premolars were used for transmission electron microscopy examination and three-dimensional (3D) reconstruction of pulpal blood vessels and their surrounding nerves. Serial Epon thin sections were taken from specimens. After a series of preprocessing steps that included digitization, contrast enhancement, slice alignment, segmentation and… (More)