K. M. J. van Laere

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An arabinofuranohydrolase (AXH-d3) was purified from a cell-free extract of Bifidobacterium adolescentis DSM 20083. The enzyme had a molecular mass of approximately 100 kDa as determined by gel filtration. It displayed maximum activity at pH 6 and 30 °C. Using an arabinoxylan-derived oligosaccharide containing double-substituted xylopyranosyl residues(More)
Two novel arabinofuranohydrolases (AXH-d3 and AXH-m23) were purified from Bifidobacterium adolescentis DSM 20083. Both enzymes were induced upon growth of Bi. adolescentis on xylose and arabinoxylan-derived oligosaccharides. They were only active with arabinoxylans and therefore denoted as arabinoxylan arabinofuranohydrolases. Their optimal activity was at(More)
Bifidobacterium adolescentis, a gram-positive saccharolytic bacterium found in the human colon, can, alongside other bacteria, utilise stachyose in vitro thanks to the production of an α-galactosidase. The enzyme was purified from the cell-free extract of Bi. adolescentis DSM 20083T. It was found to act with retention of configuration (α→α), releasing(More)
A genomic library of Bifidobacterium adolescentis was constructed in Escherichia coli and a gene encoding an α-galactosidase was isolated. The identified open reading frame showed high similarity and identity with bacterial α-galactosidases, which belong to Family 36 of the glycosyl hydrolases. For the purification of the enzyme from the medium a single(More)
A pectic polysaccharide from soy was degraded by a crude extracellular preparation of Aspergillus aculeatus. Besides monomeric sugars, an unknown oligosaccharide was produced, which was purified and identified as the dimer β-Xyl p -(1,3)-GalA p . The enzyme responsible for the release of this dimer was purified and characterized as an exogalacturonase,(More)
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