K. L. Satya-Prakash

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Two human cell lines were established from untreated squamous cell carcinomas of the head and neck. Line 183 was derived from a head and neck squamous cell carcinoma of the tonsil and 1483 from a head and neck squamous cell carcinoma of the retromolar trigone. Both lines grow in a cobblestone pattern demonstrating their epithelial heritage.(More)
The chromosomal constitution of 8 human breast tumor cell lines has been analyzed by conventional staining and G-banding methods. The stem line number was established in each case. In all cell lines, a large number of marker chromosomes have been identified. In addition to the 1q marker chromosome, previously reported to be present in several breast tumors(More)
Application of the silver-staining technique to air-dried chromosome preparations of the yellow fever mosquito, Aedes aegypti, revealed the following: 1) intensely stained pericentromeric regions in all chromosomes including the Y chromosome in spermatogonia, and during the primary and secondary spermatocytes; 2) the presence of prepachytene that were not(More)
A simple method has been described for the visualization of chromosome cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mitotic chromosomes in all the materials(More)
A silver-staining technique revealed the core morphology of metaphase chromosomes of irradiated CHO cells with chromatid lesions (breaks, gaps). These cells were photographed before and after silver staining. As a rule, the core was not continuous in chromatid gaps, suggesting that the chromatid is broken in many so-called gaps. Ten cytogeneticists who were(More)
Metaphases of Chinese hamster cells in culture, when blocked by mitotic arrestants, regardless of their mechanisms, would recover when a proper concentration was used. Arrested metaphases would quickly enter anaphase and karyokinesis ensued. However, the frequency of abnormal anaphases would increase when the period of arrest was prolonged, and the(More)
The behavior of the sex chromosomes during meiosis in the house shrew, Suncus murinus, shows some interesting features. Both X- and Y-chromosomes are large and biarmed and have huge segments of C-band material in noncentromeric areas. A distinct chiasma is formed between the short arms of the X and Y chromosomes and the heterochromatic regions in the(More)
Most human and animal carcinogens induce gene mutation, chromosome breakage or other types of DNA lesions. However, recent studies indicate that some carcinogens do not directly damage DNA, but may cause missegregation of chromosomes resulting in aneuploidy production. Aneuploidy-producing agents pose serious genetic hazards to the human population. Such(More)
Two rare de novo cases are presented of pediatric erythroleukemia (EL), AML-M6 in a four-month-old (patient A) and four-year-old (patient B) African-Americans who presented to the Medical College of Georgia from 1989 to 1995. The clinical, morphologic, immunophenotypic and cytogenetic features of both patients are reviewed. The purpose of this study is to(More)