K L Satya-Prakash

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Two human cell lines were established from untreated squamous cell carcinomas of the head and neck. Line 183 was derived from a head and neck squamous cell carcinoma of the tonsil and 1483 from a head and neck squamous cell carcinoma of the retromolar trigone. Both lines grow in a cobblestone pattern demonstrating their epithelial heritage.(More)
The chromosomal constitution of 8 human breast tumor cell lines has been analyzed by conventional staining and G-banding methods. The stem line number was established in each case. In all cell lines, a large number of marker chromosomes have been identified. In addition to the 1q marker chromosome, previously reported to be present in several breast tumors(More)
A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mitotic chromosomes in all the materials(More)
Most human and animal carcinogens induce gene mutation, chromosome breakage or other types of DNA lesions. However, recent studies indicate that some carcinogens do not directly damage DNA, but may cause missegregation of chromosomes resulting in aneuploidy production. Aneuploidy-producing agents pose serious genetic hazards to the human population. Such(More)
Metaphases of Chinese hamster cells in culture, when blocked by mitotic arrestants, regardless of their mechanisms, would recover when a proper concentration was used. Arrested metaphases would quickly enter anaphase and karyokinesis ensued. However, the frequency of abnormal anaphases would increase when the period of arrest was prolonged, and the(More)
The objective of this study was to test the concept that the G1 period lacks any specific function in the life cycle of mammalian cells and hence could be drastically reduced without any effect on the generation time. HeLa cells were grown in medium containing an optimum dose (60 microM) of hydroxyurea at which the duration of S period was prolonged with(More)
Application of the silver-staining technique to air-dried chromosome preparations of the yellow fever mosquito, Aedes aegypti, revealed the following: 1) intensely stained pericentromeric regions in all chromosomes including the Y chromosome in spermatogonia, and during the primary and secondary spermatocytes; 2) the presence of prepachytene that were not(More)
The objective of this study was to determine whether transformed cells have greater DNA synthesis-inducing ability (DSIA) than normal cells when fused with G1 phase cells. HeLa cells synchronized in G1 phase, prelabeled with large latex beads, were fused separately with (a) quiescent human diploid fibroblasts (HDF), (b) HDF partially synchronized in late(More)
A silver-staining technique revealed the core morphology of metaphase chromosomes of irradiated CHO cells with chromatid lesions (breaks, gaps). These cells were photographed before and after silver staining. As a rule, the core was not continuous in chromatid gaps, suggesting that the chromatid is broken in many so-called gaps. Ten cytogeneticists who were(More)