Learn More
The E. coli secE (prlG) gene codes for an integral cytoplasmic membrane protein which is part of the cell's secretory machinery. A deletion of nearly the entire gene renders the cell dependent on the presence of a complementing secE+ plasmid, indicating that the SecE protein is essential for growth. Deletions which remove carboxy-terminal sequences or(More)
Two general approaches have been used to define genetically the genes that encode components of the cellular protein export machinery. One of these strategies identifies mutations that confer a conditional-lethal, pleiotropic export defect (sec, secretion). The other identifies dominant suppressors of signal sequence mutations (prl, protein localization).(More)
The pseudoautosomal region of the human X and Y chromosomes is subject to frequent X-Y recombination during male meiosis. We report the finding of two pseudoautosomal loci, DXYS20 and DXYS28, characterized by highly informative restriction fragment length polymorphisms (RFLPs). The pseudoautosomal character of DXYS20 and DXYS28 was formally demonstrated by(More)
Strains of Escherichia coli in which lacZ (specifies beta-galactosidase) is fused to genes that specify exported proteins such as LamB (lambda receptor) exhibit unusual phenotypes. In particular, such strains are killed by high-level expression of the LacZ hybrid protein. Previous results suggest that this overproduction phenotype is the consequence of a(More)
Three strategies for genetic analysis show that two inner membrane components of the export machinery, PrlA (SecY) and PrlG (SecE), interact directly while catalyzing the translocation of secreted proteins across the cytoplasmic membrane of E. coli. The first, suppressor-directed inactivation (SDI), exploits the specific interaction between dominant prl(More)
Genetic studies have identified six genes whose products comprise the general protein secretion machinery of Escherichia coli. Insights from mutant analysis and the biochemical properties of the purified components allows the secretion pathway to be described in some detail. The picture emerging provides a useful paradigm for similar pathways in other(More)
  • 1