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A nonparametric method of discriminant analysis is proposed. It is based on nonparametric extensions of commonly used scatter matrices. Two advantages result from the use of the proposed nonparametric scatter matrices. First, they are generally of full rank. This provides the ability to specify the number of extracted features desired. This is in contrast… (More)
A nonparametric data reduction technique is proposed. Its goal is to select samples that are ``representative'' of the entire data set. The technique is iterative and is based on the use of a criterion function and nearest neighbor density estimates. Experiments are presented to demonstrate the algorithm.
As an experiment for the application of knowledge-based techniques to large-scale software maintenance, a program called PROMPTER which produces annotations for programs written in an assembly language is being developed. PROMPTER adopts object-oriented approach and represents both hardware and programming knowledge as class/instance definitions.… (More)
Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available premade phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This paper… (More)
The site-specific introduction of a haloacetamide derivative into a designated cysteine on a displaying peptide on a capsid protein (gp10) of bacteriophage T7 has been achieved. This easiest gp10-based thioetherification (10BASEd-T) is carried out in one-pot without side reactions or loss of phage infectivity.
A number of properties of separable covariance matrices are summarized. Expressions for the divergence of the corresponding two-dimensional Gaussian random processes are given in terms of row and column covariance matrices, and in terms of linear prediction parameters and maximum likelihood spectral estimates. Such time and frequency domain expressions are… (More)
We have achieved site-specific conjugation of several haloacetamide derivatives into designated cysteines on bacteriophage T7-displayed peptides, which are fused to T7 capsid protein gp10. This easiest gp10 based-thioetherification (10BASEd-T) undergoes almost quantitatively like a click reaction without side reaction or loss of phage infectivity. The… (More)
By using the 10BASEd-T, we have synthesized a crown ether-like macrocyclic library possessing randomized peptide linkers on bacteriophage T7. Among 1.5 × 10(9) diversities of the supramolecule candidates, we have obtained a specific binder for the N-terminal domain of Hsp90.