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We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used(More)
Patch-clamp recording from the apical surface of the epithelium of frog lens reveals a cation-selective channel after pressure (about +/- 30 mm Hg) is applied to the pipette. The open state of this channel has a conductance of some 50 pS near the resting potential (-56.1 +/- 2.3 mV) when 107 mM NaCl and 10 HEPES (pH 7.3) is outside the channel. The(More)
Intracranial pressure (ICP) was measured continuously in anesthetized, free-breathing, adult, male Sprague-Dawley rats and New Zealand White rabbits by means of a subarachnoid screw technique. The effect upon ICP of changing the volumes within the cranium by infusion of artificial cerebrospinal fluid into the lateral cerebral ventricle at various rates was(More)
We have reviewed the evidence in favor of a prostaglandin mediator of the thermal responses in fever and found that PGE injected into the hypothalamus does not always cause fever, that cerebrospinal fluid concentrations of PGE are not reliable reflections of hypothalamic events, and that antipyretic drugs may act in ways other than inhibiting PGE synthesis.(More)
paper, selected historical aspects of thermoregulation and fever are presented as background to the application of molecular biology to thermoreg-ulation. Temperature-sensing mechanisms, coordination of thermal information , thermoregulatory circuitry, efferent responses to thermal stimuli, set point mechanisms, and some of the mechanisms and consequences(More)
Vasopressin administered into the ventral septum exerts a dose-related antipyresis. This site of action is similar in a number of species. The fever-reducing properties of vasopressin are both site and neuropeptide specific. Evidence supporting a role for endogenous vasopressin in fever suppression is the demonstration that the release of the peptide from(More)
The possibility that arginine vasopressin (AVP) is involved in the etiology of febrile convulsions was investigated by experiments on hyperthermia-induced convulsions in rats. Homozygous Brattleboro rats, which genetically lack AVP, and Long Evans rats, which were passively immunized by intracerebroventricular anti-AVP antiserum, either convulsed at higher(More)
In the study of mechanisms of thermoregulation conscious animals are used and in most cases the conscious animal is the preferred choice. In some investigations however an anesthetized animal may be the model necessary for the practical purposes of the experimental design. We have developed, tested, and used an urethane anesthetized animal as a model in(More)
Voltage-gated, tetrodotoxin(TTX)-blockable sodium channels are found in most excitable cells and are the primary contributors to action potentials generated by many of these cells. To date, there has only been one report of a non-cultured vertebrate epithelial cell type containing TTX-blockable Na+ channels: rabbit non-pigmented ciliary body epithelial(More)
The effect of an antipyretic drug administered directly into the preoptic-anterior hypothalamus was measured in order to investigate the role of fever on mortality of bacterially infected mammals. New Zealand white rabbits (Oryctolagus cuniculus) were injected intravenously with Pasteurella multocida and either sodium salicylate or a control solution was(More)