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Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.

• K Bastyns, Sabine Chapelle, Patrick Vandamme, H J Goossens, Rien De Wachter
• Molecular and cellular probes
• 1995

The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like… (More)

Acrylamide quenching of the fluorescence of glyceraldehyde-3-phosphate dehydrogenase: reversible and irreversible effects.

• K Bastyns, Yves Engelborghs
• Photochemistry and photobiology
• 1992

The acrylamide quenching of the tryptophan fluorescence of apo and holo glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was studied. In the case of apo-GAPDH, the steady state fluorescence quenching… (More)

Experimental and theoretical study of electrostatic effects on the isoelectric pH and the pKa of the catalytic residue His-102 of the recombinant ribonuclease from Bacillus amyloliquefaciens…

• K Bastyns, Mathy Froeyen, J. F. Diaz, G. Volckaert, Yves Engelborghs
• Proteins
• 1996

Barnase, the guanine specific ribonuclease of Bacillus amyloliquefaciens, was subjected to mutations in order to alter the electrostatic properties of the enzyme. Ser-85 was mutated into Glu with the… (More)

The role of Glu-60 in the specificity of the recombinant ribonuclease from Bacillus amyloliquefaciens (barnase) towards dinucleotides, poly(A) and RNA.

• K Bastyns, M Froeyer, G. Volckaert, Yves Engelborghs
• The Biochemical journal
• 1994

A computer model of the complex between G2'p5'G and barnase, the recombinant ribonuclease of Bacillus amyloliquefaciens, was constructed, based on the known structure of the complex RNAase… (More)

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