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There has been little investigation of bacteria as gene delivery vectors. Here, we demonstrate that genetically engineered Salmonella have many of the desirable properties of a delivery vector, including targeting of multiple tumors from a distant inoculation site, selective replication within tumors, tumor retardation, and the ability to express effector(More)
A general role for chaperonin ring structures in mediating folding of newly translated proteins has been suggested. Here we have directly examined the role of the E. coli chaperonin GroEL in the bacterial cytoplasm by production of temperature-sensitive lethal mutations in this essential gene. After shift to nonpermissive temperature, the rate of general(More)
Strains in which the E. coli SOS system is continuously induced, in the absence of mutagenic treatment, have been used to generate nonsense mutations in the lacl gene. The examination of over 600 independently occurring amber and ochre mutations reveals that inducing the SOS system stimulates specifically G:C----T:A and, to a lesser extent, A:T----T:A(More)
During differentiation, human embryonic stem cells (hESCs) shut down the regulatory network conferring pluripotency in a process we designated pluripotent state dissolution (PSD). In a high-throughput RNAi screen using an inclusive set of differentiation conditions, we identify centrally important and context-dependent processes regulating PSD in hESCs,(More)
Local Ternary Pattern (LTP) is usually applied for texture classification problems. In this work, we propose LTP for human gait characterization for the purpose of human identification. Our proposed method is based on the Gait Energy Image (GEI) whereby edge information over a complete gait cycle is extracted. However, GEI does not contain enough human body(More)
Human re-identification is to match a pair of humans appearing in different cameras with non-overlapping views. However, in order to achieve this task, we need to overcome several challenges such as variations in lighting, viewpoint, pose and colour. In this paper, we propose a new approach for person re-identification in multi-camera networks by using a(More)
BACKGROUND Pathogens tolerate stress conditions that include low pH, oxidative stress, high salt and high temperature in order to survive inside and outside their hosts. Lipopolysaccharide (LPS), which forms the outer-leaflet of the outer membrane in Gram-negative bacteria, acts as a permeability barrier. The lipid A moiety of LPS anchors it to the outer(More)
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