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Thin stillage (TS), a wastewater from rice wine distillery can well sustain the growth of Gluconacetobacter xylinus for production of bacterial cellulose (BC). When used as a supplement to the traditional BC production medium (Hestrin and Schramm medium), the enhancement of BC production increased with the amount of TS supplemented in a static culture of G.(More)
Pichia pastoris has been used to produce various recombinant proteins under high oxygen demand conditions. To improve the heterologous production of β-galactosidase, the vgb gene encoding Vitreoscilla hemoglobin (VHb) was co-expressed in the P. pastoris cytoplasm under the control of the methanol-inducible promoter. Co-expression of VHb under different(More)
An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb(More)
Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and β-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell β-galactosidase activity to 4-fold higher than that of(More)
Expression of the gene (daao) encoding D-amino acid oxidase (DAAO) in Escherichia coli typically results in a marked decrease of cell viability, and it has generally been assumed that the consumption of intracellular D-alanine by DAAO is responsible for this effect. Vitreoscilla hemoglobin (VHb) gene (vgb) was coexpressed with Rhodosporidium toruloides(More)
This paper reports a study in automatic sentiment classification, i.e., automatically classifying documents as expressing positive or negative sentiments. The study investigates the effectiveness of using a machine-learning algorithm, support vector machine (SVM), on various text features to classify on-line product reviews into recommended (positive(More)
We demonstrate a thermoelectric nanogenerator (NG) made from a single Sb-doped ZnO micro/nanobelt that generates an output power of about 1.94 nW under a temperature difference of 30 K between the two electrodes. A single Sb-doped ZnO microbelt was bonded at its ends on a glass substrate as a NG, which can give an output voltage of 10 mV and an output(More)
  • Jyh Ming Wu, Cheng-Ying Chen, +5 authors Zhong Lin Wang
  • 2012
We demonstrated a flexible strain sensor based on ZnSnO(3) nanowires/microwires for the first time. High-resolution transmission electron microscopy indicates that the ZnSnO(3) belongs to a rhombohedral structure with an R3c space group and is grown along the [001] axis. On the basis of our experimental observation and theoretical calculation, the(More)
We have developed a simple, cost-effective, and scalable approach to fabricate a piezoelectric nanogenerator (NG) with stretchable and flexible characteristics using BaTiO3 nanotubes, which were synthesized by the hydrothermal method. The NG was fabricated by making a composite of the nanotubes with polymer poly(dimethylsiloxane) (PDMS). The peak(More)
We demonstrated a single microwire photodetector first made using a VO2 microwire that exhibted high responsivity (Rλ) and external quantum efficiency (EQE) under varying light intensities. The VO2 nanowires/microwires were grown and attached on the surface of the SiO2/Si(100) substrate. The SiO2 layer can produce extremely low densities of long VO2(More)