Justin M Ingram

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Electrophysiological recordings show intense neuronal firing during epileptic seizures leading to enhanced energy consumption. However, the relationship between oxygen metabolism and seizure patterns has not been well studied. Recent studies have developed fast and quantitative techniques to measure oxygen microdomain concentration during seizure events. In(More)
Dynamic analysis of oxygen (O₂) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer(More)
19 Electrophysiological recordings show intense neuronal firing during epileptic seizures leading to enhanced 20 energy consumption. However, the relationship between oxygen metabolism and seizure patterns has not 21 been well studied. Recent studies have developed fast and quantitative techniques to measure oxygen 22 microdomain concentration during(More)
22 We utilized a novel ratiometric nano quantum dot fluorescence resonance energy transfer (NQD-FRET) 23 optical sensor to quantitatively measure oxygen dynamics from single cell microdomains during hypoxic 24 episodes as well as during 4-aminopyridine (4AP) induced spontaneous seizure-like events in rat hip25 pocampal slices. Coupling oxygen sensing with(More)
We utilized a novel ratiometric nanoquantum dot fluorescence resonance energy transfer (NQD-FRET) optical sensor to quantitatively measure oxygen dynamics from single cell microdomains during hypoxic episodes as well as during 4-aminopyridine (4-AP)-induced spontaneous seizure-like events in rat hippocampal slices. Coupling oxygen sensing with electrical(More)
* Correspondence: yxw170@psu.edu Center for Neural Engineering, Department of Engineering Science and Mechanics, Penn State University, PA 16802 USA Full list of author information is available at the end of the article Figure 1 Membrane potentials (top trace), extracellular potassium concentration (middle trace), and oxygen density (bottom trace) from a(More)
Short-term synaptic facilitation occurs during high-frequency stimulation, is known to be dependent on presynaptic calcium ions, and persists for tens of milliseconds after a presynaptic action potential. We have used the frog neuromuscular junction as a model synapse for both experimental and computer simulation studies aimed at testing various mechanistic(More)
25 26 Short-term synaptic facilitation occurs during high frequency stimulation, is known to be dependent on 27 presynaptic calcium ions, and persists for tens of milliseconds after a presynaptic action potential. We 28 have used the frog neuromuscular junction as a model synapse for both experimental and computer 29 simulation studies aimed at testing(More)
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