Justin D. Smith

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Supercentenarians (110 years or older) are the world's oldest people. Seventy four are alive worldwide, with twenty two in the United States. We performed whole-genome sequencing on 17 supercentenarians to explore the genetic basis underlying extreme human longevity. We found no significant evidence of enrichment for a single rare protein-altering variant(More)
Measuring natural selection on genomic elements involved in the cis-regulation of gene expression--such as transcriptional enhancers and promoters--is critical for understanding the evolution of genomes, yet it remains a major challenge. Many studies have attempted to detect positive or negative selection in these noncoding elements by searching for those(More)
We present an approach for implementing language-level primitives for whole-process migration and speculative execution in a compiler and associated runtime environment. These primitives are exposed to the user through simple language constructs that do not require the user to manage process state explicitly. With migration and speculation we show how the(More)
Coercive family processes are germane to the development of problem behaviors in early childhood, yet the cognitive and affective underpinnings are not well understood. We hypothesized that one antecedent of early coercive interactions is the caregiver's implicit affective attitudes toward the child, which in this article are termed relational schemas.(More)
This study examines the association between adolescents' relational schemas and their subjective understanding of interactions in the context of male-female romantic relationships. We employed an innovative multimodal methodology: the video-recall system [Welsh, D. P., & Dickson, J. W. (2005). Video-recall procedures for examining subjective understanding(More)
Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have not been well characterized. Here, we define rules that determine gRNA effectiveness for transcriptional repression in Saccharomyces cerevisiae. We create an inducible single plasmid CRISPRi system(More)
Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for biochemical and genetic manipulation of DNA. Although complexes between Cas9 and guide RNAs (gRNAs) offer remarkable specificity and versatility for genome manipulation, mis-targeted events occur. To extend the understanding of gRNA::target homology requirements, we(More)
The valid and reliable assessment of fidelity is critical at all stages of intervention research and is particularly germane to interpreting the results of efficacy and implementation trials. Ratings of protocol adherence typically are reliable, but ratings of therapist competence are plagued by low reliability. Because family context and case(More)
Although single genes underlying several evolutionary adaptations have been identified, the genetic basis of complex, polygenic adaptations has been far more challenging to pinpoint. Here we report that the budding yeast Saccharomyces paradoxus has recently evolved resistance to citrinin, a naturally occurring mycotoxin. Applying a genome-wide test for(More)
BACKGROUND To improve the quality, quantity, and speed of implementation, careful monitoring of the implementation process is required. However, some health organizations have such limited capacity to collect, organize, and synthesize information relevant to its decision to implement an evidence-based program, the preparation steps necessary for successful(More)