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Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open(More)
Hirsutella rhossiliensis and H. minnesotensis are endoparasitic fungi of the second-stage juvenile (J2) of the soybean cyst nematode (Heterodera glycines) in nature. They also parasitize both H. glycines J2 and Caenorhabditis elegans on agar plates. Agrobacterium tumefaciens-mediated transformation conditions were established for these Hirsutella spp. The(More)
The objective of the present study was to investigate the optimal nutritional requirements for mycelial growth and sporulation of entomopathogenic fungus Aschersonia aleyrodis Webber by orthogonal layout methods. Herein the order of effects of nutrient components on mycelial growth was tryptone > Ca(2+) > soluble starch > folacin, corresponding to the(More)
The culture media for mycelial growth and sporulation of the entomopathogenic fungus Aschersonia placenta were optimized using the response surface method (RSM). Interactions of medium components and the optimization of a biphasic production system were studied using Box-Behnken design (BBD) with three levels of three variables. Experimentation confirmed(More)
The fungi Hirsutella rhossiliensis and Hirsutella minnesotensis generally parasitize only plant-parasitic nematodes in nature but parasitize the bacterivorous nematode Caenorhabditis elegans on agar plates. To establish a model system for studying the interaction between fungi and nematodes, we compared the parasitism of the first- to fourth-stage larvae(More)
A, a spore of AS3G1 (a H. minnesotensis transformant expressing ZsGreen) attached to H. glycines second stage juvenile (J2); B, formation of an infection peg; C, the apex of the infection peg becoming globose; D, initial colonization of coelom by hyphae; E–G, hyphal growth through coelom; H–J, hyphal growth out of coelom; K–L, formation of new conidia. The(More)
The fungus Aschersonia placenta FJSM was evaluated for control of the sweet potato whitefly, Bemisia tabaci. Bemisia tabaci nymphs (1st-4th instars) on tomato plants in the greenhouse (25-27 °C, 70%-85% relative humidity) were sprayed with suspensions containing 0, 10(4), 10(5), 10(6), 10(7), or 10(8) A. placenta FJSM conidia/mL. Mortality of fungus-treated(More)
This report describes the isolation of a new azaphilone, designated hypocrellone A (2), together with five known compounds (1, 3-6) from a submerged culture of the entomopathogenic fungus Hypocrella sp. (isolate WYTY-21). The absolute stereostructures of the two compounds (1 and 2) were elucidated based on 1D and 2D NMR spectroscopic and mass spectrometric(More)
The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based on the response surface method (RSM).(More)
The infection of insects by the entomopathogenic fungus Aschersonia placenta depends on conidia. To identify proteins differentially expressed in A. placenta conidia vs mycelia, we performed a comparative proteomic analysis of A. placenta using 2-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization/time-of-flight mass(More)