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A proteomics approach to understanding protein ubiquitination
TLDR
A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination. Expand
Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome.
TLDR
The combination of strong cation exchange (SCX) and reversed-phase (RP) chromatography to achieve two-dimensional separation prior to MS/MS and 1,504 yeast proteins were unambiguously identified in this single analysis. Expand
Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins
TLDR
The molecular requirements for LAP are characterized, and Rubicon is identified as being required for L AP but not autophagy, thereby elucidating the importance of LAP in response to A. fumigatus infection. Expand
Quantitative Proteomics Reveals the Function of Unconventional Ubiquitin Chains in Proteasomal Degradation
TLDR
It is reported that the unconventional linkages are abundant in vivo and that all non-K63 linkages may target proteins for degradation, and that unconventional polyubiquitin chains are critical for ubiquitin-proteasome system function. Expand
NUDEL Is a Novel Cdk5 Substrate that Associates with LIS1 and Cytoplasmic Dynein
TLDR
NUDEL is a novel LIS1-interacting protein with sequence homology to gene products also implicated in nuclear distribution in fungi and is a substrate of Cdk5, a kinase known to be critical during neuronal migration. Expand
Identification of Neuronal RNA Targets of TDP-43-containing Ribonucleoprotein Complexes*♦
TLDR
This work reveals the protein and RNA components of the TDP-43-containing ribonucleoprotein complexes and provides a framework for understanding how dysregulation of T DP-43 in RNA metabolism contributes to neurodegeneration. Expand
C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles
TLDR
It is found that arginine-containing DPRs, polyGly-Arg (GR) and polyPro- Arg (PR), interact with RNA-binding proteins and proteins with low complexity sequence domains (LCDs) that often mediate the assembly of membrane-less organelles. Expand
Semiquantitative Proteomic Analysis of Rat Forebrain Postsynaptic Density Fractions by Mass Spectrometry*
TLDR
A comprehensive analysis of purified PSD fractions by liquid chromatography coupled with tandem mass spectrometry reveals crucial information about molecular abundance as well as molecular diversity in the PSD, and provides a basis for further studies on the molecular mechanisms of synaptic function and plasticity. Expand
MicroRNA miR‐137 Regulates Neuronal Maturation by Targeting Ubiquitin Ligase Mind Bomb‐1
TLDR
A novel miRNA‐mediated mechanism involving miR‐137 and Mib1 that function to regulate neuronal maturation and dendritic morphogenesis during development is demonstrated. Expand
Autophosphorylated CaMKIIα Acts as a Scaffold to Recruit Proteasomes to Dendritic Spines
TLDR
It is reported that CaMKIIalpha-an abundant postsynaptic protein kinase-mediates the activity-dependent recruitment of proteasomes to dendritic spines in hippocampal neurons, revealing a scaffolding role of post Synaptic CaMKIalpha in activity- dependent proteasome redistribution, which is commensurate with the great abundance of CaMK IIalpha in synapses. Expand
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