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A proteomics approach to understanding protein ubiquitination
A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination.
Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome.
- Junmin Peng, J. Elias, Carson C. Thoreen, L. Licklider, S. Gygi
- BiologyJournal of proteome research
The combination of strong cation exchange (SCX) and reversed-phase (RP) chromatography to achieve two-dimensional separation prior to MS/MS and 1,504 yeast proteins were unambiguously identified in this single analysis.
Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins
The molecular requirements for LAP are characterized, and Rubicon is identified as being required for L AP but not autophagy, thereby elucidating the importance of LAP in response to A. fumigatus infection.
NUDEL Is a Novel Cdk5 Substrate that Associates with LIS1 and Cytoplasmic Dynein
Quantitative Proteomics Reveals the Function of Unconventional Ubiquitin Chains in Proteasomal Degradation
C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles
Identification of Neuronal RNA Targets of TDP-43-containing Ribonucleoprotein Complexes*♦
This work reveals the protein and RNA components of the TDP-43-containing ribonucleoprotein complexes and provides a framework for understanding how dysregulation of T DP-43 in RNA metabolism contributes to neurodegeneration.
Semiquantitative Proteomic Analysis of Rat Forebrain Postsynaptic Density Fractions by Mass Spectrometry*
- Junmin Peng, M. J. Kim, Dongmei Cheng, D. Duong, S. Gygi, M. Sheng
- BiologyJournal of Biological Chemistry
- 14 May 2004
A comprehensive analysis of purified PSD fractions by liquid chromatography coupled with tandem mass spectrometry reveals crucial information about molecular abundance as well as molecular diversity in the PSD, and provides a basis for further studies on the molecular mechanisms of synaptic function and plasticity.
Lysine Propionylation and Butyrylation Are Novel Post-translational Modifications in Histones*S
It is confirmed, by in vitro labeling and peptide mapping by mass spectrometry, that two previously known acetyltransferases, p300 and CREB-binding protein, could catalyze lysine propionylation and lysines butyrylation in histones.
Tat Modifies the Activity of CDK9 To Phosphorylate Serine 5 of the RNA Polymerase II Carboxyl-Terminal Domain during Human Immunodeficiency Virus Type 1 Transcription
The studies suggest that the ability of Tat to increase transcriptional elongation may be due to its ability to modify the substrate specificity of the CDK9 complex.