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Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells
TLDR
PTD.AdeGFP significantly improved gene transfer to multiple cell types deficient in expression of CAR and was able to transduce target cells in a dynamin-independent pathway, which would be valuable in gene-function analysis and for gene therapy in cancer.
Aptazyme-mediated direct modulation of post-transcriptional sgRNA level for conditional genome editing and gene expression.
TLDR
A novel system was established to regulate the post-transcriptional sgRNA level by small molecule-controlled aptazyme, which was shown to reduce the off-target effects caused by Cas9/sgRNA, while enabling precise temporal control over gene editing and regulatory activity.
A Highly Sensitive Sandwich ELISA to Detect CSF Progranulin: A Potential Biomarker for CNS Disorders.
TLDR
Results indicate that this ELISA system can be valuable in studying the diagnostic and prognostic value of CSF PGRN in brain disorders and could monitor CNS metastasis and CSF seeding of carcinomas.
Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene
TLDR
A novel luciferase knock-in reporter system is generated, which will be very useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SRE BP1 gene expression.
Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout
TLDR
An in vitro hepatic steatosis cell model was established by knockout of the DGKθ gene in liver cancer cell line HepG2 using CRISPR/Cas9 technology, providing a valuable tool for understanding the pathogenesis of nonalcoholic fatty liver disease and associated insulin resistance, and for developing treatment strategies for this disease.
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