Junichi Homma

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Toxoids of protease and elastase of Pseudomonas aeruginosa were successfully prepared by treatment with 8% formalin plus 0.2 m lysine and by 4% formalin respectively. The two toxoids proved sufficiently potent to elicit high antibody titers as estimated by both the enzyme-neutralizing and passive hemagglutination tests. The effectiveness of immunizing minks(More)
Biological and antiviral activities of chemically synthesized lipid A-subunit analogues, GLA-27 and GLA-60, were investigated with respect to defense mechanisms such as macrophage and natural killer (NK) cell activation and interferon (IFN)-inducing activity. GLA-27, a 4-O-phosphono-D-glucosamine derivative carrying 3-O-tetradecanoyl (C14) and(More)
To confirm the revised lipid A structure of Escherichia coli and to establish the structure responsible for its functions, biological activities of the synthetic compounds based on the presented structure of E. coli lipid A were investigated. Compound 506, 2-deoxy-6-O-(2-deoxy-2-[(R)-3-dodecanoyloxytetradecanoylamino]-3-O(More)
Comparative studies on the virulence of two strains of Pseudomonas aeruginosa isolated from otic pus and mink pneumonic lesion, respectively, were made by means of intranasal inoculation in mink. The minimal lethal dose of the former (strain NC-5, highly virulent for mice) was 10(10), and that of the latter (strain No. 5, moderately virulent for mice) was(More)
Antimetastatic effects of the monosaccharic lipid A-subunit analogue GLA-60 against B16-F10 melanomas were investigated. Intravenous injection of 10 micrograms GLA-60 one day or two days before transplantation of 10(5) melanoma cells resulted in a significant decrease in the number of melanoma colonies metastasized into the lung. Intramuscular injection was(More)
Protease and elastase were inoculated intracutaneously in rabbits, the process of which was followed. Of the enzymes, 50 mug caused circumscribed hemorrhage at the inoculated local and subcutaneous part, and ulcerating lesions of skin and extensive hemorrhagic lesion at the subcutaneous tissues were observed in the case of inoculation with 250 mug. The(More)
A chemically synthesized lipid A-subunit analogue, GLA-60, 2-deoxy-4-O-phosphono-2-[(3R)-3-hydroxytetradecanamido]-3-O-[(3R)- 3- tetradecanoyloxytetradecanoyl]-D-glucose, has many of the activities of endotoxin but has little toxicity. Then, compounds with various lengths of acyl side chain of the acyloxyacyl group at the 3-O position of GLA-60 were(More)
Chemiluminescence response upon phagocytosis was found to be enhanced in circulating blood monocytes derived from patients with active sarcoidosis, suggesting that these monocytes are activated and easily generate reactive species of oxygen including singlet oxygen. Such enhancement was observed even when patients were receiving high doses of(More)