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Activation of rod photoreceptors by light induces a massive redistribution of the heterotrimeric G-protein transducin. In darkness, transducin is sequestered within the membrane-enriched outer segments of the rod cell. In light, it disperses throughout the entire neuron. We show here that redistribution of rod transducin by light requires activation, but it(More)
Arrestins ensure the timely termination of receptor signaling. The role of rhodopsin phosphorylation in visual arrestin binding was established more than 20 years ago, but the effects of the number of receptor-attached phosphates on this interaction remain controversial. Here we use purified rhodopsin fractions with carefully quantified content of(More)
Structural features of neurons create challenges for effective production and distribution of essential metabolic energy. We investigated how metabolic energy is distributed between cellular compartments in photoreceptors. In avascular retinas, aerobic production of energy occurs only in mitochondria that are located centrally within the photoreceptor. Our(More)
Although methyltransferase has been recognized as a major element that governs the epigenetic regulation of the genome during temozolomide (TMZ) chemotherapy in glioblastoma multiforme (GBM) patients, its regulatory effect on glioblastoma chemoresistance has not been well defined. This study investigated whether DNA methyltransferase (DNMT) expression was(More)
Additive manufacturing (AM) technologies have been successfully applied in various applications. Fused deposition modeling (FDM), one of popular AM techniques, is most widely used method for manufacturing of plastic materials. Due to the poor strength properties of pure plastic materials, there is a critical need to improve mechanical properties for(More)
Extended Abstract Low-energy collision-induced dissociation (CID) of peptides in a collision cell has been introduced as a fast method for peptide sequencing [1]. The method has been adapted to the analysis of gel-isolated proteins by the introduction of the nanoESI technology [2]. In the meantime proteins can be identified by search-engine supported(More)
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