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The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution microscopy and present a comprehensive evaluation of localization software packages. Our philosophy is(More)
Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present(More)
We propose pretreatment using microneedles to increase perianal skin permeability for locally targeted delivery of phenylephrine (PE), a drug that increases resting anal sphincter pressure to treat fecal incontinence. Microneedle patches were fabricated by micromolding poly-lactic-acid. Pre-treatment of human cadaver skin with microneedles increased PE(More)
A highly sensitive and specific RNA biosensor was developed for the rapid detection of viable Escherichia coli as an indicator organism in water. The biosensor is coupled with protocols developed earlier for the extraction and amplification of mRNA molecules from E. coli [Anal. Biochem. 303 (2002) 186]. However, in contrast to earlier detection methods, the(More)
Purpose: Due to the potential risk of inducing cancers, radiation dose of X-ray CT should be reduced for routine patient scanning. However, in low-dose X-ray CT, severe artifacts usually occur due to photon starvation, beamhardening, etc, which decrease the reliability of diagnosis. Thus, high quality reconstruction from low-dose X-ray CT data has become(More)
Functionalizing surface enhanced the molecular sensing ability of a fabricated nanopore by increasing the translocation duration time for a short double-stranded DNA. The surface of nanopore was derivatized with gamma-aminopropyltriethoxysilane and the positively charged surface attracted DNA molecules when they were in the vicinity of nanopore. The(More)
A highly sensitive and specific assay method was developed for the detection of viable Escherichia coli as an indicator organism in water, using nucleic acid sequence-based amplification (NASBA) and electrochemiluminescence (ECL) analysis. Viable E. coli were identified via a 200-nt-long target sequence from mRNA (clpB) coding for a heat shock protein. In(More)
A functional biosurface applicable to a biomemory device was fabricated using ferritin, which is one of the globular protein complexes consisting of 24 protein subunits, which can be classified as metalloproteins. For the fabrication of uniform ferritin layer, 11-MUA(11-mercaptoundecanoic acid) was used as a linker material. The formation of the ferritin(More)
2010 WILEY-VCH Verlag Gm Over the last 30 years, advances in electrical and chemical engineering in integrated-circuit technologies for memory and information processors have provided microcomputing machines with ever more powerful processing capabilities. However, the conventional scaling methods used in the semiconductor industry face increasing(More)
Localization microscopy such as STORM/PALM achieves the super-resolution by sparsely activating photoswitchable probes. However, to make the activation sparse enough to obtain reconstruction images using conventional algorithms, only small set of probes need to be activated simultaneously, which limits the temporal resolution. Hence, to improve temporal(More)