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Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present(More)
A highly sensitive and specific assay method was developed for the detection of viable Escherichia coli as an indicator organism in water, using nucleic acid sequence-based amplification (NASBA) and electrochemiluminescence (ECL) analysis. Viable E. coli were identified via a 200-nt-long target sequence from mRNA (clpB) coding for a heat shock protein. In(More)
A protein-based electrochemical sensor for hydrogen peroxide (H(2)O(2)) was developed by an easy and effective film fabrication method where spinach ferredoxin (Fdx) containing [2Fe-2S] metal center was cross linked with 11-mercaptoundecanoic acid (MUA) on a gold (Au) surface. The surface morphology of Fdx molecules on Au electrodes was investigated by(More)
A highly sensitive and specific RNA biosensor was developed for the rapid detection of viable Escherichia coli as an indicator organism in water. The biosensor is coupled with protocols developed earlier for the extraction and amplification of mRNA molecules from E. coli [Anal. Biochem. 303 (2002) 186]. However, in contrast to earlier detection methods, the(More)
This study described the prospect of silica nanotube surface modification in simultaneous detection of pathogenic bacteria by coupling cation exchange magnetic separation with quantum dot labeling. The outer surface of magnetic nanoparticles embedded long silica nanotube (capturing SNT) was modified with poly-L-lysine to serve as a cation exchange magnetic(More)
We propose pretreatment using microneedles to increase perianal skin permeability for locally targeted delivery of phenylephrine (PE), a drug that increases resting anal sphincter pressure to treat fecal incontinence. Microneedle patches were fabricated by micromolding poly-lactic-acid. Pre-treatment of human cadaver skin with microneedles increased PE(More)
The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution microscopy and present a comprehensive evaluation of localization software packages. Our philosophy is(More)
A functional biosurface applicable to a biomemory device was fabricated using ferritin, which is one of the globular protein complexes consisting of 24 protein subunits, which can be classified as metalloproteins. For the fabrication of uniform ferritin layer, 11-MUA(11-mercaptoundecanoic acid) was used as a linker material. The formation of the ferritin(More)
—Localization microscopy such as STORM/PALM achieves the super-resolution by sparsely activating photo-switchable probes. However, to make the activation sparse enough to obtain reconstruction images using conventional algorithms, only small set of probes need to be activated simultaneously, which limits the temporal resolution. Hence, to improve temporal(More)