Learn More
TGA transcription factors are implicated as regulators of pathogenesis-related (PR) genes because of their physical interaction with the known positive regulator, nonexpresser of PR gene1 (NPR1). A triple-knockout mutant tga2-1 tga5-1 tga6-1 was shown previously to be defective in the induction of PR genes and systemic acquired resistance, confirming their(More)
RAD52 protein has an important role in homology-directed DNA repair by mediating RAD51 nucleoprotein filament formation on single-stranded DNA (ssDNA) protected by replication protein-A (RPA) and annealing of RPA-coated ssDNA. In human, cellular response to DNA damage includes phosphorylation of RAD52 by c-ABL kinase at tyrosine 104. To address how this(More)
Named Data Networking (NDN) uses content names as routing entries, and thus the scalability of NDN routing is of primary concern. NDN allows in-network caching as a built-in functionality; however, if network nodes make caching decisions individually, duplicate copies of the same content may exist among nearby nodes. To address these problems, we propose(More)
λ exonuclease degrades one strand of duplex DNA in the 5'-to-3' direction to generate a 3' overhang required for recombination. Its ability to hydrolyze thousands of nucleotides processively is attributed to its ring structure, and most studies have focused on the processive phase. Here we have used single-molecule fluorescence resonance energy transfer(More)
Phosphates along the DNA function as chemical energy frequently used by nucleases to drive their enzymatic reactions. Exonuclease functions as a machine that converts chemical energy of the phosphodiester-chain into mechanical work. However, the roles of phosphates during exonuclease activities are unknown. We employed λ exonuclease as a model system and(More)
A dual arm master-slave servo-manipulator system has been developed for use in a pyroprocess integrated demonstration facility at Korea Atomic Energy Research Institute. The slave manipulator with a reach of 1.6 m can handle a 25 kgf payload in any posture, where the gravity force of remote tools or handling equipment has a great impact on the position(More)
Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate(More)
  • 1