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The present study focuses on characterization of two hemicellulases, RuXyn1 and RuXyn2, from rumen bacterial metagenome and their capabilities for degradation of xylans. Glycosyl hydrolase (GH) family 43 β-d-xylosidase/α-l-arabinofuranosidase RuXyn1 can hydrolyze p-nitrophenyl-β-d-xylopyranoside (pNPX), p-nitrophenyl-α-l-arabinofuranoside (pNPA), and(More)
Alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of biochemicals and renewable biofuels. This study described the isolation of a new alginate metabolizing bacterium, Flavobacterium sp. S20, from sludge samples and the characterization of its alginate lyase Alg2A. The alginate lyase gene,(More)
The thermostability of xylanase XynR8 from uncultured Neocallimastigales rumen fungal was improved by combining random point mutagenesis with site-directed mutagenesis guided by rational design, and a thermostable variant, XynR8_VNE, was identified. This variant contained three amino acid substitutions, I38V, D137N and G151E, and showed an increased melting(More)
The work environment of control unit is not very well for intelligent circuit breaker, such as electromagnetic interference is one important factor. In this paper, hardware and software were applied as anti-Interference measures to ensure the Electromagnetic Compatibility (EMC), so the reliability of circuit breaker system was advanced. The external and(More)
Some algorithms of close degree in fuzzy pattern recognition were researched and their limitations in application were found. So a new algorithm of close degree wais proposed based on fluctuation thought in this paper. The whole relation between various elements and fuzzy set was considered in the new algorithm. It could avoid that the little change of(More)
The system reliability is determined by both failure mode and reliability of system units. Therefore, the assessment of system reliability must firstly be depended on the assessment of the reliability of system units. In the same reliability system, the system reliability performance will be affected by the composition of different system unites. In(More)
Product failure distribution research is a basic research for product advanced design and manufacture, and has an important role in intelligent computing. Traditional analysis of the failure distribution about small samples has some limitations, for example, the weibull distribution test is better as the sample size is more than 10; the Figure test is(More)
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