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Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density(More)
MicroRNA (miR)390 cleaves the non-coding TAS3 precursor RNA for the production of tasiRNA-ARF, a group of an endogenous trans-acting small-interfering RNAs which cleave the transcripts of auxin response factor (ARF) 3/4. miR390-cleaved TAS3 RNA is polymerized and diced into tasiRNA-ARF by RNA-dependent RNA polymerase6 (RDR6) and Dicer-like4 (DCL4),(More)
Aim Understanding how ecological and evolutionary processes together determine patterns of biodiversity remains a central aim in biology. Guided by ecological theory, we use data from multiple arthropod lineages across the Hawaiian archipelago to explore the interplay between ecological (population dynamics, dispersal, trophic interactions) and evolutionary(More)
Rice has the unique ability to express alpha-amylase under anoxic conditions, a feature that is critical for successful anaerobic germination and growth. Previously, anaerobic conditions were shown to up-regulate the expression of Amy3 subfamily genes (Amy3B/C, 3D, and 3E) in rice embryos. These genes are known to be feedback regulated by the hydrolytic(More)
Many therapeutic proteins have been launched in market or gone into development stages for their high therapeutic efficacy. The proteins can be developed mainly as liquid or solid dosage forms; pre-filled syringes or freeze-dried. Regardless of the dosage forms, they have several stability issues due to the intrinsic properties of the proteins, which can(More)
The viscosity of highly concentrated protein solutions was evaluated using lysozyme as model protein. Viscosity profiles of lysozyme were examined with the effect of buffer and pH-value at various concentrations. The viscosity of lysozyme dissolved in water increased continuously with the concentration as the slope of shear stress against shear rate(More)
The Arabidopsis thaliana root system, which comprises primary, lateral, and adventitious roots, responds differentially to environmental and hormonal signals. For example, exposure to high concentrations of the plant hormone auxin promotes lateral root (LR) initiation and emergence; however, high levels of auxin inhibit primary root (PR) growth. Here, we(More)
Controlling the production of diverse cell/tissue types is essential for the development of multicellular organisms such as animals and plants. The Arabidopsis thaliana root, which contains distinct cells/tissues along longitudinal and radial axes, has served as an elegant model to investigate how genetic programs and environmental signals interact to(More)
Ca2+ as in other cellular signaling pathways plays a key signal-mediating function in plant immune responses. However, a negative immune activity of some Ca2+-binding calmodulins implicates a rather confusing function of Ca2+ despite its importance in plant immunity. We therefore studied here an effect of Ca2+ on Arabidopsis disease resistance to an adapted(More)
  • Al Nadaf, Deakin Je, Gilbert C, Robinson Tj, Pd A, Albrecht Cf +25 others
  • 2013
species comparison of escape from X inactivation in Eutheria: implications for evolution of X chromosome inactivation. MAKUNGA NP. LC-MS-based metabolomics assists with quality assessment and traceability of wild and cultivated plants of Sutherlandia frutescens (Fabeaceae). Sticky plant captures prey for symbiotic bug: is this digestive mutualism?. Effect(More)