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In this paper, a corner block list -- a new efficient topological representation for non-slicing floorplan is proposed with applications to VLSI floorplan and building block placement. Given a corner block list, it takes only linear time to construct the floorplan. Unlike the O-tree structure, which determines the exact floorplan based on given block sizes,(More)
Human cytochrome P450 2A13 (CYP2A13), which is highly efficient in the metabolic activation of a major tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), may play important roles in xenobiotic toxicity and tobacco-related tumorigenesis in the respiratory tract. The aim of this study was to identify any genetic polymorphisms(More)
To assess the importance of brain cytochrome P450 (P450) activity in mu opioid analgesic action, we generated a mutant mouse with brain neuron-specific reductions in P450 activity; these mice showed highly attenuated morphine antinociception compared with controls. Pharmacological inhibition of brain P450 arachidonate epoxygenases also blocked morphine(More)
A mouse model with liver-specific deletion of the NADPH-cytochrome P450 reductase (Cpr) gene (designated Alb-Cre/Cprlox mice) was generated and characterized in this study. Hepatic microsomal CPR expression was significantly reduced at 3 weeks and was barely detectable at 2 months of age in the Alb-Cre+/-/Cprlox+/+ (homozygous) mice, with corresponding(More)
A Gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain SS011B1-4(T), was isolated from benthic sediment of the South China Sea. Optimum growth occurred at 30-37 degrees C, pH 7.5-8.0 and 4-8 % (w/v) NaCl. Strain SS011B1-4(T) utilized a variety of organic substrates as sole carbon(More)
AIM To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis. METHODS The methylation specific PCR in conjunction of sequencing verification was used to establish the methylation-profile of the promoter CpG islands of 31 genes in colorectal cancer (n = 65), the neighboring(More)
Acetaminophen overdose causes toxicity in liver and extrahepatic tissues. Although it is well established that cytochrome P450 enzymes play a critical role in the metabolic activation of acetaminophen, it is not yet clear whether acetaminophen toxicity in extrahepatic tissues is a consequence of hepatic biotransformation. The aim of this study was to(More)
Two strategies of metabolic engineering have been used, individually or combined, to alter the metabolic flux to improve the production of S-adenosyl-L-methionine (SAM) in Pichia pastoris. One is over expressing SAM synthase by knock-in technique, the other is the disruption of cystathionine-beta synthase (CBS) by knock-out technique. Strain Gsam with(More)
The herbicide 2,6-dichlorobenzonitril (DCBN) is a potent and tissue-specific toxicant to the olfactory mucosa (OM). The toxicity of DCBN is mediated by cytochrome P450 (P450)-catalyzed bioactivation; however, it is not known whether target-tissue metabolic activation is essential for toxicity. CYP2A5, expressed abundantly in both liver and OM, was(More)