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OBJECTIVE To determine whether the preantral follicles in adult ovaries can generate developmentally competent oocytes after in vitro culture. DESIGN Prospective, animal-model study. SETTING Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Seoul, Korea. ANIMAL(S) B6CBAF1 mice. INTERVENTION(S) Preantral follicles collected(More)
Primary follicles retrieved from B6CBAF1 prepubertal mice were cultured in a stepwise manner in an alpha-minimum essential medium-based medium to generate viable embryos and embryonic stem cell (ESC)-like cells. A significant increase in follicle growth and oocyte maturation accompanied by increased secretion of 17beta-estradiol and progesterone was(More)
Graphene/carbon nanotube (CNT) hybrid structures are fabricated for use as optical arrays. Vertically aligned CNTs are directly synthesized on a graphene/quartz substrate using plasma-enhanced chemical vapor deposition (PECVD). Graphene preserves the transparency and resistance during CNT growth. Highly aligned single-walled CNTs show a better performance(More)
This study was conducted to evaluate if mouse preantral follicles can yield developmentally competent oocytes following culture in serum-free, defined medium. Donor follicles were obtained from 14-day-old B6CBAF1 mice, and cultured in α-MEM-Glutamax medium. The replacement of fetal bovine serum with knockout serum replacement (KSR) did not significantly(More)
With the advances in the separation and purification of carbon nanotubes (CNTs), the use of highly pure metallic or semiconducting CNTs has practical merit in electronics applications. When highly pure CNTs are applied in various fields, CNT networks are preferred to individual CNTs. In such cases, the presence of an electrical path becomes crucial in the(More)
Stability and high energy densities are essential qualities for emerging battery electrodes. Because of its high specific capacity, silicon has been considered a promising anode candidate. However, the several-fold volume changes during lithiation and delithiation leads to fractures and continuous formation of an unstable solid-electrolyte interphase (SEI)(More)
To study the genomic plasticity of somatic cells without ectopic genetic manipulation, we cultured mouse fibroblasts with ovarian cells, embryonic fibroblasts of different strains, and parthenogenetic embryonic stem cells (ESCs). Of 41 trials, cell aggregation resembling nascent ESC colony from inner cell mass was detected in 9 cases (22%), and 6 cases(More)
We report on the fabrication of high-quality GaN on soda-lime glass substrates, heretofore precluded by both the intolerance of soda-lime glass to the high temperatures required for III-nitride growth and the lack of an epitaxial relationship with amorphous glass. The difficulties were circumvented by heteroepitaxial coating of GaN on ZnO nanorods via a(More)
Since Advanced Encryption Standard (AES) in stream modes, such as counter (CTR), output feedback (OFB) and cipher feedback (CFB), can meet most industrial requirements, the range of applications for dedicated stream ciphers is decreasing. There are many attack results using algebraic properties and side channel information against stream ciphers for(More)
temperature. This wide spectral range enables potential applications from the infrared to the deep ultraviolet regions of the electromagnetic spectrum. This makes GaN-based nitrides one of the most important materials for light sources such as light-emitting diodes (LEDs). [ 1–5 ] Sapphire has long been used as a substrate for GaN-based LEDs, and silicon(More)