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Förster Resonance Energy Transfer (FRET) measured with Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique to investigate spatio-temporal regulation of protein-protein interactions in living cells. When using standard fitting methods to analyze time domain FLIM, the correct estimation of the FRET parameters requires a high number of(More)
Fö rster Resonance Energy Transfer (FRET) measured with Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique to investigate spatio-temporal regulation of protein-protein interactions in living cells. When using standard fitting methods to analyze time domain FLIM, the correct estimation of the FRET parameters requires a high number of(More)
Genetically encoded Förster Resonance Energy Transfert (FRET) biosensors are powerful tools for monitoring spatiotemporal biochemical activities in living samples. By labelling a probe protein with a pair of fluorescent proteins, FRET measurement allows to follow a conformational change of the probe sensor to a specific activity. A very exciting challenge(More)
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