Julie W. Meadows

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The sulfonamide resistance gene from plasmid R46 encodes for a mutated dihydropteroate synthase insensitive to inhibition by sulfonamides. Its coding sequence was fused to the pea ribulose bisphosphate carboxylase/oxygenase transit peptide sequence. Incubation of isolated chloroplasts with the fusion protein synthesised in vitro, showed that the bacterial(More)
Increased prostaglandin E(2)(PGE(2)) synthesis involves multiple enzymes and two isoforms of the terminal enzyme of this biosynthetic pathway, PGE synthase (PGES), were recently identified. Cytosolic PGES (cPGES) is identical to the Hsp90 chaperone, p23, and is reportedly functionally coupled to constitutive PG endoperoxide H synthase-1 (PGHS-1). Microsomal(More)
The 33 kd protein of the photosynthetic oxygen-evolving complex is synthesized in the cytoplasm as a larger precursor and transported into the thylakoid lumen via a stromal intermediate form. In this report we describe a reconstituted system in which the later stages of this import pathway can be studied in isolation. We demonstrate import of the 33 kd(More)
A full-length cDNA encoding the precursor of the lumenal 33 kDa oxygen-evolving complex protein from wheat was inserted into a prokaryotic expression vector. Cell-free transcription-translation of this construct generates a precursor protein of the correct size. However, when expressed in Escherichia coli, the protein is quantitatively exported into the(More)
The light-driven oxidation of water in the thylakoid membrane is carried out by photosystem II, a multisubunit complex containing both integral and extrinsic components. The wateroxidation reaction takes place on the lumenal side of the membrane and is catalysed by a group of proteins, often termed the oxygen-evolving complex, of which three proteins of 33,(More)
A chimaeric gene was constructed encoding the pre-sequence of the 33 kDa oxygen-evolving complex protein from wheat (a thylakoid lumen protein) linked to ricin A chain. The fusion protein is efficiently imported by isolated pea chloroplasts and localised partly in the stroma, with the remainder bound to the stromal surface of the thylakoids. The imported(More)
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