Julie Sunderland

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A rapid high-performance liquid chromatography (HPLC) assay for the detection of linezolid in human serum was developed. The method used a Hypersil 5ODS stationary phase. The mobile phase was 1% ortho-phosphoric acid, 30% methanol, 2 g/L heptane sulphonic acid, pH 5. UV absorbance detection was used (lambda(max) 254 nm). Samples were prepared by mixing with(More)
OBJECTIVE The effect of a single 5 day enrofloxacin treatment on the native Campylobacter coli population in conventionally weaned 5-week-old pigs was investigated. MATERIALS Twelve pigs were split into two groups of six: one group was treated with a therapeutic dose (15 mg/pig/day) of enrofloxacin and the other remained untreated to act as the control.(More)
AIMS To investigate the effect of a therapeutic and sub-therapeutic chlortetracycline treatment on tetracycline-resistant Salmonella enterica serovar Typhimurium DT104 and on the commensal Escherichia coli in pig. METHODS AND RESULTS Salmonella Typhimurium DT104 was orally administered in all pigs prior to antibiotic treatment, and monitored with the(More)
BACKGROUND The purpose of this study is to evaluate how wetting of Collatamp (a gentamicin-containing collagen implant [GCCI]) impacts on the gentamicin content of the implant and whether this affects its potential antibacterial efficacy. METHODS GCCI (Collatamp(®), EUSA Pharma [Europe], Oxford, United Kingdom) containing 130 mg gentamicin and 280 mg(More)
A reverse-phase HPLC assay is described for the simultaneous assay of enrofloxacin (ENR) and ciprofloxacin (CPX) in pig faeces. Extraction used dichloromethane, 2-propanol and 0.3M ortho-phosphoric acid (1:5:4 v/v/v). Separation was achieved using a Spherisorb S5 C8 column, heated to 50 degrees C and a mobile phase of 0.16% ortho-phosphoric acid (adjusted(More)
The conditions under which noscapine causes high levels of polyploidy in vitro in human lymphocytes were investigated to try to determine its mode of action and to assess whether it was likely to be a genotoxic hazard when used as an antitussive agent. Irrespective of duration of treatment or type of medium, there seemed to be a threshold for polyploidy(More)
Sir, Linezolid, a new oxazolidinone, has a unique mode of action.1 The antimicrobial agent has a wide spectrum of activity against Gram-positive organisms, including methicillinresistant Staphylococcus aureus,2,3 penicillin-resistant pnemococci3 and both vancomycin-resistant Enterococcus faecalis and Enterococcus faecium.3,4 Linezolid is available in oral(More)
The importance of polyploidy as a genotoxic lesion is uncertain and there have been few publications and no reviews which have included data on spontaneous or induced polyploidy in routine genotoxicity screening. We have attempted to clarify some of the issues by reviewing the published literature and by reference to our historical data base for metaphase(More)