Most E3 ligases use a RING domain to activate a thioester-linked E2∼ubiquitin-like protein (UBL) intermediate and promote UBL transfer to a remotely bound target protein. Nonetheless, RING E3 mechanisms matching a specific UBL and acceptor lysine remain elusive, including for RBX1, which mediates NEDD8 ligation to cullins and >10% of all ubiquitination. We… (More)
Erroneous kinetochore-microtubule interactions must be detected and corrected before a cell enters anaphase to prevent chromosome mis-segregation. Two new studies describe an Aurora A-mediated error correction mechanism based on the spatial position of a chromosome within the mitotic spindle.