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Biosecurity issues associated with current and emerging embryo technologies.
A variety of procedures associated with in vivo and in vitro embryo production, as well as cloning and transgenics, are in current use by both researchers and practitioners. Biohazards associatedExpand
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Infectivity of bovine viral diarrhea virus associated with in vivo-derived bovine embryos.
Early research indicated that bovine viral diarrhea virus (BVDV) would not adhere to zona pellucida-intact (ZP-I), in vivo-derived bovine embryos. However, in a recent study, viral association ofExpand
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Different strains of noncytopathic bovine viral diarrhea virus (BVDV) vary in their affinity for in vivo-derived bovine embryos.
Washing procedures (without trypsin treatment) recommended by the International Embryo Transfer Society (IETS) for use on in vivo-derived embryos effectively removed a cytopathic strain (NADL) ofExpand
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Bovine viral diarrhea virus in embryo and semen production systems.
Although BVDV-free offspring have been produced from persistently infected bulls and heifers via advanced reproductive techniques, embryos and semen can potentially transmit the virus. Due to thisExpand
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Seroconversion of calves following intravenous injection with embryos exposed to bovine viral diarrhea virus (BVDV) in vitro.
Two recent studies demonstrated that a high-affinity isolate of BVDV (SD-1), remained associated with a small percentage of in vivo-derived bovine embryos following artificial exposure to the virusExpand
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186 SEROCONVERSION OF CALVES TO BOVINE VIRAL DIARRHEA VIRUS (BVDV) FOLLOWING INTRAVENOUS INOCULATION WITH ARTIFICIALLY EXPOSED IN VIVO-DERIVED EMBRYOS
An early study demonstrated that washing embryos was effective for removal of virus after artificial exposure of in vivo-derived embryos to a cytopathic isolate of BVDV (Singh et al., 1982Expand
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186 SEROCONVERSION OF CALVESTO BOVINEVIRAL DIARRHEAVIRUS (BVDV) FOLLOWING INTRAVENOUS INOCULATIONWITHARTIFICIALLY EXPOSED INVIVO-DERIVED EMBRYOS
13% of the calves occurred following injection with sonicate fluids from washed and trypsin-treated embryos, respectively. Findings demonstrated that the quantity of a high affinity isolate of BVDVExpand
35 PRODUCTION OF CLONED MINIATURE CALVES USING CYTOPLASTS FROM COWS OF STANDARD SIZE
Numerous genetically similar cattle for use in research or teaching can be produced with chromatin transfer technology (Sullivan et al. 2004 Biol. Reprod. 70, 146–153). Miniature cattle can provideExpand
214 Use of virus isolation and quantitative polymerase chain-reaction techniques to detect bovine viral diarrhea virus (BVDV) in single or small groups of pre-implantation bovine embryos.
Because of its broad distribution among populations of cattle and its association with materials of animal origin used in embryo production, bovine viral diarrhea virus (BVDV) is a potential problemExpand