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gamma-Aminobutyric acid (GABA)-ergic and glycinergic inhibition is believed to play a major role in the respiratory network. In the present study we tested whether specific blockade of glycinergic inhibition resulted in changes in respiratory network interaction and function. Using the working heart-brainstem preparation from adult mice, we recorded phrenic(More)
Two alternative concepts have been ooered to explain the neural mechanisms responsible for the generation of the respiratory motor pattern in the brainstem: a network paradigm and a hybrid pacemaker-network paradigm. Our computational and experimental studies were aimed at " building a bridge " between these concepts and considering the conditions that may(More)
BACKGROUND Using cell-type-specific promoters to restrict gene expression to particular cells is an attractive approach for gene therapy, but often hampered by insufficient transcriptional activity of these promoters. Previous studies have shown that transcriptional amplification strategy (TAS) can be used to enhance the activity of such promoters without(More)
The changes in motor activity of the respiratory rhythm generator were quantitatively analysed in mice (from birth to at least 56 days old) in both awake and anaesthetized preparations, as well as in vitro to define the age at which the respiratory network is mature. In awake and anaesthetized spontaneously breathing mice respiratory-related thoracic(More)
We describe a preparation of arterially-perfused spinal cord with attached hindquarters, taken from adult mouse. This is the first preparation of adult mammalian spinal cord tissue to have the advantages of an in vitro approach whilst retaining intact intraspinal circuitry, sensory inputs, and somatic and sympathetic segmental outputs. The functional(More)
The central integration of signals from pulmonary vagal C-fibers (or type-J receptors) with those arising from cardiac, peripheral chemoreceptor, and baroreceptor afferents to neurons within the nucleus of the solitary tract (NTS) was studied in an arterially perfused working heart-brain stem preparation of adult mouse. Pulmonary vagal C-fibers were excited(More)
1. The importance of glycinergic and GABAAergic synaptic mechanisms for respiratory rhythm generation in the maturing mouse were investigated in vivo and in an in vitro slice preparation generating respiratory rhythmic activity spontaneously at all postnatal ages. 2. The effect on respiration of topical application of strychnine or bicuculline to the(More)
The present report describes a novel rhythmically active brainstem slice preparation that generates respiratory activity spontaneously in both mice and rats of varying maturational states. The brainstems of neonatal (0-4 days) and mature (3-8 weeks) mice and rats were isolated and a 600- to 750-microns thick slice cut to include the dorsomedial and the(More)
1. The relationship between sympathetic and somatic motor outflows from thoraco-lumbar spinal cord was investigated in a novel arterially perfused trunk-hindquarters preparation of adult mouse. 2. Ongoing activity was present in both somatic motor (obturator, sciatic or femoral nerves) and sympathetic outflows (either renal nerve or abdominal sympathetic(More)
To study the ontogenesis of central respiratory rhythm generation, a novel brainstem slice preparation was developed that generates respiratory rhythmic activity spontaneously in mice and rats at all post-natal ages. The slice was made by tilting the brainstem to include both the ventrolateral and dorsomedial medulla. This 'tilted-sagittal' slice contained(More)